Induction of metallothionein-I (MT-I) mRNA in primary astrocyte cultures is mediated by hypotonicity and not ethanol (EtOH) per se

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Metallothionein (MT) mRNA was determined in rat astrocyte cultures in response to ethanol (EtOH). MT-I mRNA was significantly increased after 6 h exposure to isosmotic EtOH, but not hyperosmotic EtOH. Exposure to a hyposmotic/hypotonic solution also led to a significant increase in the expression of astrocytic MT-I mRNA. The large increase in MT-I mRNA was not due to removal of extracellular NaCl, because this effect was reversed by replacement of NaCl with N-methyl D-glucamine chloride. A significant decrease in MT-I mRNA was also noted in astrocytes exposed to an EtOH-free hyperosmotic/hypertonic solution. These results suggest (1) that EtOH per se does not directly induce MT-I mRNA expression, (2) that the induction by EtOH of MT-I mRNA is secondary to hypotonicity, and (3) that hyperosmotic/hypertonic exposure is associated with reduced expression of MT-I mRNA in astrocyte cultures.

Original languageEnglish (US)
Pages (from-to)289-293
Number of pages5
JournalBrain Research
Volume770
Issue number1-2
DOIs
StatePublished - Oct 3 1997
Externally publishedYes

Fingerprint

Metallothionein
Astrocytes
Ethanol
Messenger RNA
Hypotonic Solutions
Hypertonic Solutions
Chlorides

Keywords

  • Astrocyte
  • Ethanol
  • Hypotonic
  • In vitro
  • Metallothionein
  • mRNA
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Induction of metallothionein-I (MT-I) mRNA in primary astrocyte cultures is mediated by hypotonicity and not ethanol (EtOH) per se. / Aschner, Michael; Conklin, Dawn R.; Aschner, Judy L.

In: Brain Research, Vol. 770, No. 1-2, 03.10.1997, p. 289-293.

Research output: Contribution to journalArticle

@article{867d64cacd05411499328d8004655c85,
title = "Induction of metallothionein-I (MT-I) mRNA in primary astrocyte cultures is mediated by hypotonicity and not ethanol (EtOH) per se",
abstract = "Metallothionein (MT) mRNA was determined in rat astrocyte cultures in response to ethanol (EtOH). MT-I mRNA was significantly increased after 6 h exposure to isosmotic EtOH, but not hyperosmotic EtOH. Exposure to a hyposmotic/hypotonic solution also led to a significant increase in the expression of astrocytic MT-I mRNA. The large increase in MT-I mRNA was not due to removal of extracellular NaCl, because this effect was reversed by replacement of NaCl with N-methyl D-glucamine chloride. A significant decrease in MT-I mRNA was also noted in astrocytes exposed to an EtOH-free hyperosmotic/hypertonic solution. These results suggest (1) that EtOH per se does not directly induce MT-I mRNA expression, (2) that the induction by EtOH of MT-I mRNA is secondary to hypotonicity, and (3) that hyperosmotic/hypertonic exposure is associated with reduced expression of MT-I mRNA in astrocyte cultures.",
keywords = "Astrocyte, Ethanol, Hypotonic, In vitro, Metallothionein, mRNA, Rat",
author = "Michael Aschner and Conklin, {Dawn R.} and Aschner, {Judy L.}",
year = "1997",
month = "10",
day = "3",
doi = "10.1016/S0006-8993(97)00772-5",
language = "English (US)",
volume = "770",
pages = "289--293",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Induction of metallothionein-I (MT-I) mRNA in primary astrocyte cultures is mediated by hypotonicity and not ethanol (EtOH) per se

AU - Aschner, Michael

AU - Conklin, Dawn R.

AU - Aschner, Judy L.

PY - 1997/10/3

Y1 - 1997/10/3

N2 - Metallothionein (MT) mRNA was determined in rat astrocyte cultures in response to ethanol (EtOH). MT-I mRNA was significantly increased after 6 h exposure to isosmotic EtOH, but not hyperosmotic EtOH. Exposure to a hyposmotic/hypotonic solution also led to a significant increase in the expression of astrocytic MT-I mRNA. The large increase in MT-I mRNA was not due to removal of extracellular NaCl, because this effect was reversed by replacement of NaCl with N-methyl D-glucamine chloride. A significant decrease in MT-I mRNA was also noted in astrocytes exposed to an EtOH-free hyperosmotic/hypertonic solution. These results suggest (1) that EtOH per se does not directly induce MT-I mRNA expression, (2) that the induction by EtOH of MT-I mRNA is secondary to hypotonicity, and (3) that hyperosmotic/hypertonic exposure is associated with reduced expression of MT-I mRNA in astrocyte cultures.

AB - Metallothionein (MT) mRNA was determined in rat astrocyte cultures in response to ethanol (EtOH). MT-I mRNA was significantly increased after 6 h exposure to isosmotic EtOH, but not hyperosmotic EtOH. Exposure to a hyposmotic/hypotonic solution also led to a significant increase in the expression of astrocytic MT-I mRNA. The large increase in MT-I mRNA was not due to removal of extracellular NaCl, because this effect was reversed by replacement of NaCl with N-methyl D-glucamine chloride. A significant decrease in MT-I mRNA was also noted in astrocytes exposed to an EtOH-free hyperosmotic/hypertonic solution. These results suggest (1) that EtOH per se does not directly induce MT-I mRNA expression, (2) that the induction by EtOH of MT-I mRNA is secondary to hypotonicity, and (3) that hyperosmotic/hypertonic exposure is associated with reduced expression of MT-I mRNA in astrocyte cultures.

KW - Astrocyte

KW - Ethanol

KW - Hypotonic

KW - In vitro

KW - Metallothionein

KW - mRNA

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0030726984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030726984&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(97)00772-5

DO - 10.1016/S0006-8993(97)00772-5

M3 - Article

VL - 770

SP - 289

EP - 293

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -