Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

B. F. Mackler, L. C. Altman, David L. Rosenstreich, J. J. Oppenheim

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines1, and are implicated in facilitating antibody responses2. In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)3. We have now found that the erythrocyte-antibody (19S)-complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.

Original languageEnglish (US)
Pages (from-to)834-837
Number of pages4
JournalNature
Volume249
Issue number5460
DOIs
StatePublished - 1974
Externally publishedYes

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Lymphokines
Lymphocyte Activation
Mitogens
B-Lymphocytes
Antibodies
Macrophage Migration-Inhibitory Factors
T-Lymphocytes
Cellular Immunity
Thymus Gland
Blood Cells
Guinea Pigs
Erythrocytes
Bone Marrow
Lymphocytes
Membranes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation. / Mackler, B. F.; Altman, L. C.; Rosenstreich, David L.; Oppenheim, J. J.

In: Nature, Vol. 249, No. 5460, 1974, p. 834-837.

Research output: Contribution to journalArticle

Mackler, B. F. ; Altman, L. C. ; Rosenstreich, David L. ; Oppenheim, J. J. / Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation. In: Nature. 1974 ; Vol. 249, No. 5460. pp. 834-837.
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