Induction of histone acetylation and inhibition of growth of mouse erythroleukemia cells by S-allylmercaptocysteine

Michael A. Lea, Mariam Rasheed, Verrell M. Randolph, Faika Khan, Asif Shareef, Charles DesBordes

Research output: Contribution to journalArticle

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Abstract

Growth-inhibitory effects on DS19 mouse erythroleukemia cells were seen in the micromolar concentration range with allicin and S-allylmercaptocysteine and in the millimolar range with allyl butyrate, allyl phenyl sulfone, and S-allyl cysteine. Increased acetylation of histones was induced by incubation of cells with the allyl compounds at concentrations similar to those that resulted in the inhibition of cell proliferation. The induction of histone acetylation by S-allylmercaptocysteine was also observed in Caco-2 human colon cancer cells and T47D human breast cancer cells. In contrast to the effect on histone acetylation, there was a decrease in the incorporation of phosphate into histones when DS19 cells were incubated with 25 μM Sallylmercaptocysteine. Histone deacetylase activity was inhibited by allyl butyrate, but there was little or no effect with the allyl sulfur compounds examined in this study. A similar degree of downregulation of histone deacetylase and histone acetyltransferase was observed when DS19 cells were incubated with S-allylmercaptocysteine or allyl isothiocyanate. The induction of histone acetylation by S-allylmercaptocysteine was not blocked by a proteasome inhibitor. The mechanism by which S-allylmercaptocysteine induces histone acetylation remains to be characterized. It may be related in part to metabolism to allyl mercaptan, which is a more effective inhibitor of histone deacetylase.

Original languageEnglish (US)
Pages (from-to)90-102
Number of pages13
JournalNutrition and Cancer
Volume43
Issue number1
StatePublished - 2002
Externally publishedYes

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Leukemia, Erythroblastic, Acute
acetylation
Acetylation
histones
Histones
growth retardation
histone deacetylase
Allyl Compounds
mice
Growth
Histone Deacetylases
Butyrates
cells
butyrates
allyl sulfur compounds
allicin
allyl isothiocyanate
Histone Acetyltransferases
Sulfur Compounds
Proteasome Inhibitors

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Oncology

Cite this

Lea, M. A., Rasheed, M., Randolph, V. M., Khan, F., Shareef, A., & DesBordes, C. (2002). Induction of histone acetylation and inhibition of growth of mouse erythroleukemia cells by S-allylmercaptocysteine. Nutrition and Cancer, 43(1), 90-102.

Induction of histone acetylation and inhibition of growth of mouse erythroleukemia cells by S-allylmercaptocysteine. / Lea, Michael A.; Rasheed, Mariam; Randolph, Verrell M.; Khan, Faika; Shareef, Asif; DesBordes, Charles.

In: Nutrition and Cancer, Vol. 43, No. 1, 2002, p. 90-102.

Research output: Contribution to journalArticle

Lea, MA, Rasheed, M, Randolph, VM, Khan, F, Shareef, A & DesBordes, C 2002, 'Induction of histone acetylation and inhibition of growth of mouse erythroleukemia cells by S-allylmercaptocysteine', Nutrition and Cancer, vol. 43, no. 1, pp. 90-102.
Lea, Michael A. ; Rasheed, Mariam ; Randolph, Verrell M. ; Khan, Faika ; Shareef, Asif ; DesBordes, Charles. / Induction of histone acetylation and inhibition of growth of mouse erythroleukemia cells by S-allylmercaptocysteine. In: Nutrition and Cancer. 2002 ; Vol. 43, No. 1. pp. 90-102.
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