Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis

Eleanor Roy; A. Dharshan De Silva; Vasan K. Sambandamurthy; Simon O. Clark; Evangelos Stavropoulos; William R. Jacobs; John Brennan; John Chan; Ann Williams; M. Joseph Colston; Ricardo E. Tascon

doi:10.1016/j.imlet.2005.10.011

Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis

Eleanor Roy, A. Dharshan De Silva, Vasan K. Sambandamurthy, Simon O. Clark, Evangelos Stavropoulos, William R. Jacobs, John Brennan, John Chan, Ann Williams, M. Joseph Colston, Ricardo E. Tascon

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4⁺ and CD8⁺ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.

Original language	English (US)
Pages (from-to)	196-199
Number of pages	4
Journal	Immunology Letters
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/j.imlet.2005.10.011
State	Published - Mar 15 2006

Keywords

Auxothrophic
Dendritic
Protective
Tuberculosis
Vaccine

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.imlet.2005.10.011

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Roy, E., De Silva, A. D., Sambandamurthy, V. K., Clark, S. O., Stavropoulos, E., Jacobs, W. R., Brennan, J., Chan, J., Williams, A., Colston, M. J., & Tascon, R. E. (2006). Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. Immunology Letters, 103(2), 196-199. https://doi.org/10.1016/j.imlet.2005.10.011

Roy, E, De Silva, AD, Sambandamurthy, VK, Clark, SO, Stavropoulos, E, Jacobs, WR, Brennan, J, Chan, J, Williams, A, Colston, MJ & Tascon, RE 2006, 'Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis', Immunology Letters, vol. 103, no. 2, pp. 196-199. https://doi.org/10.1016/j.imlet.2005.10.011

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title = "Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis",

abstract = "Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4+ and CD8+ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.",

keywords = "Auxothrophic, Dendritic, Protective, Tuberculosis, Vaccine",

author = "Eleanor Roy and {De Silva}, {A. Dharshan} and Sambandamurthy, {Vasan K.} and Clark, {Simon O.} and Evangelos Stavropoulos and Jacobs, {William R.} and John Brennan and John Chan and Ann Williams and Colston, {M. Joseph} and Tascon, {Ricardo E.}",

note = "Funding Information: We thank The Medical Research Council for Support and for funding Eleanor Roy. Work done at AECOM was supported by NIH grants AI 26170 and HL 71241. We also thank Dr. Joe Locker for assistance with the histopathological work, Dr. Brigitta Stockinger for advice and for providing us with the immortalised tsDC cell line used in the guinea pigs studies and to B. Chen for his technical assistance.",

year = "2006",

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day = "15",

doi = "10.1016/j.imlet.2005.10.011",

language = "English (US)",

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pages = "196--199",

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T1 - Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis

AU - Roy, Eleanor

AU - De Silva, A. Dharshan

AU - Sambandamurthy, Vasan K.

AU - Clark, Simon O.

AU - Stavropoulos, Evangelos

AU - Jacobs, William R.

AU - Brennan, John

AU - Chan, John

AU - Williams, Ann

AU - Colston, M. Joseph

AU - Tascon, Ricardo E.

N1 - Funding Information: We thank The Medical Research Council for Support and for funding Eleanor Roy. Work done at AECOM was supported by NIH grants AI 26170 and HL 71241. We also thank Dr. Joe Locker for assistance with the histopathological work, Dr. Brigitta Stockinger for advice and for providing us with the immortalised tsDC cell line used in the guinea pigs studies and to B. Chen for his technical assistance.

PY - 2006/3/15

Y1 - 2006/3/15

N2 - Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4+ and CD8+ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.

AB - Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4+ and CD8+ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.

KW - Auxothrophic

KW - Dendritic

KW - Protective

KW - Tuberculosis

KW - Vaccine

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Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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