Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis

Eleanor Roy, A. Dharshan De Silva, Vasan K. Sambandamurthy, Simon O. Clark, Evangelos Stavropoulos, William R. Jacobs, John Brennan, John Chan, Ann Williams, M. Joseph Colston, Ricardo E. Tascon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adoptively transferred dendritic cells presenting antigens derived from different pathogens have been shown to elicit specific T cell responses and to induce protective antibacterial immunity. We describe here the induction of high levels of protective immunity in mice using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. We provide evidence that protection is superior to BCG and that it is associated with increased priming of CD4+ and CD8+ T cells specific for mycobacterial antigens. This method for generating high levels of anti-bacterial protective immunity could be helpful in the design of novel vaccines against tuberculosis and other intracellular pathogens.

Original languageEnglish (US)
Pages (from-to)196-199
Number of pages4
JournalImmunology Letters
Volume103
Issue number2
DOIs
StatePublished - Mar 15 2006

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Mycobacterium tuberculosis
Dendritic Cells
Immunity
Vaccination
Tuberculosis Vaccines
T-Lymphocytes
Antigens
Mycobacterium bovis

Keywords

  • Auxothrophic
  • Dendritic
  • Protective
  • Tuberculosis
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. / Roy, Eleanor; De Silva, A. Dharshan; Sambandamurthy, Vasan K.; Clark, Simon O.; Stavropoulos, Evangelos; Jacobs, William R.; Brennan, John; Chan, John; Williams, Ann; Colston, M. Joseph; Tascon, Ricardo E.

In: Immunology Letters, Vol. 103, No. 2, 15.03.2006, p. 196-199.

Research output: Contribution to journalArticle

Roy, E, De Silva, AD, Sambandamurthy, VK, Clark, SO, Stavropoulos, E, Jacobs, WR, Brennan, J, Chan, J, Williams, A, Colston, MJ & Tascon, RE 2006, 'Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis', Immunology Letters, vol. 103, no. 2, pp. 196-199. https://doi.org/10.1016/j.imlet.2005.10.011
Roy, Eleanor ; De Silva, A. Dharshan ; Sambandamurthy, Vasan K. ; Clark, Simon O. ; Stavropoulos, Evangelos ; Jacobs, William R. ; Brennan, John ; Chan, John ; Williams, Ann ; Colston, M. Joseph ; Tascon, Ricardo E. / Induction of high levels of protective immunity in mice after vaccination using dendritic cells infected with auxotrophic mutants of Mycobacterium tuberculosis. In: Immunology Letters. 2006 ; Vol. 103, No. 2. pp. 196-199.
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