Induction of alkaline phosphatase activity in cultured human intracranial tumor cells

N. Takahara, F. Herz, R. M. Singer, A. Hirano, L. G. Koss

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Alkaline phosphatase activity in several cultured primary human intracranial tumor cells varied over a relatively wide range, and there was no correlation between specific activity and the type of tumor from which the cultures were derived. The enzyme was thermolabile, and its activity was strongly inhibited by l-bromotetramisole, levamisole, and L-homoarginine but not by L-phenylalanine and L-phenylalanylglycylglycine. These are the characteristics of the liver-bone-kidney form of alkaline phosphatase. Prednisolone induced increased levels of enzyme activity in most cultures, and sodium butyrate acted as an inducer in cultures of pituitary adenoma and hemangioblastoma cells. The increase was most pronounced when responsive cells were exposed to both stimuli simultaneously. The induced alkaline phosphatase had the same properties as the enzyme of cells grown in the absence of inducers. Increased alkaline phosphatase activity was not induced by osmolality changes of the culture medium; this feature appears to be characteristic of cells producing the liver-bone-kidney enzyme form.

Original languageEnglish (US)
Pages (from-to)563-568
Number of pages6
JournalCancer Research
Volume42
Issue number2
StatePublished - 1982
Externally publishedYes

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Alkaline Phosphatase
Enzymes
Neoplasms
Homoarginine
Hemangioblastoma
Kidney
Bone and Bones
Levamisole
Butyric Acid
Liver
Pituitary Neoplasms
Prednisolone
Phenylalanine
Osmolar Concentration
Culture Media

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Takahara, N., Herz, F., Singer, R. M., Hirano, A., & Koss, L. G. (1982). Induction of alkaline phosphatase activity in cultured human intracranial tumor cells. Cancer Research, 42(2), 563-568.

Induction of alkaline phosphatase activity in cultured human intracranial tumor cells. / Takahara, N.; Herz, F.; Singer, R. M.; Hirano, A.; Koss, L. G.

In: Cancer Research, Vol. 42, No. 2, 1982, p. 563-568.

Research output: Contribution to journalArticle

Takahara, N, Herz, F, Singer, RM, Hirano, A & Koss, LG 1982, 'Induction of alkaline phosphatase activity in cultured human intracranial tumor cells', Cancer Research, vol. 42, no. 2, pp. 563-568.
Takahara N, Herz F, Singer RM, Hirano A, Koss LG. Induction of alkaline phosphatase activity in cultured human intracranial tumor cells. Cancer Research. 1982;42(2):563-568.
Takahara, N. ; Herz, F. ; Singer, R. M. ; Hirano, A. ; Koss, L. G. / Induction of alkaline phosphatase activity in cultured human intracranial tumor cells. In: Cancer Research. 1982 ; Vol. 42, No. 2. pp. 563-568.
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