Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea

Michael E T I Boerrigter, Erik Mullaart, Frits Berends, Jan Vijg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-N-nitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16% (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h post-treatment no further disappearance was observed. Inter-individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3% (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [3H]-thymidine. The cytosine analog 1-β-D-arabinofuranosyl-cytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalCarcinogenesis
Volume12
Issue number1
StatePublished - Jan 1991
Externally publishedYes

Fingerprint

Ethylnitrosourea
DNA Breaks
Lymphocytes
Blood
Proof by induction
DNA
Cytosine
Repair
Individual Differences
Phytohemagglutinins
Individuality
DNA Repair
Correlate
Thymidine
Half line
Cell Survival
Cells
Vary
Filter
Sampling

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea. / Boerrigter, Michael E T I; Mullaart, Erik; Berends, Frits; Vijg, Jan.

In: Carcinogenesis, Vol. 12, No. 1, 01.1991, p. 77-82.

Research output: Contribution to journalArticle

Boerrigter, Michael E T I ; Mullaart, Erik ; Berends, Frits ; Vijg, Jan. / Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea. In: Carcinogenesis. 1991 ; Vol. 12, No. 1. pp. 77-82.
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abstract = "We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-N-nitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16{\%} (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h post-treatment no further disappearance was observed. Inter-individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3{\%} (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [3H]-thymidine. The cytosine analog 1-β-D-arabinofuranosyl-cytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.",
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