Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea

Michael E.T.I. Boerrigter; Erik Mullaart; Frits Berends; Jan Vijg

doi:10.1093/carcin/12.1.77

Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea

Michael E.T.I. Boerrigter, Erik Mullaart, Frits Berends, Jan Vijg

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13 Scopus citations

Abstract

We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-Nnitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16% (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h posttreatment no further disappearance was observed. Inter individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3% (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [³H]- thymidine. The cytosine analog 1-β-D-arabinofuranosylcytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.

Original language	English (US)
Pages (from-to)	77-82
Number of pages	6
Journal	Carcinogenesis
Volume	12
Issue number	1
DOIs	https://doi.org/10.1093/carcin/12.1.77
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus subject areas

Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/carcin/12.1.77

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title = "Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea",

abstract = "We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-Nnitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16% (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h posttreatment no further disappearance was observed. Inter individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3% (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [3H]- thymidine. The cytosine analog 1-β-D-arabinofuranosylcytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.",

author = "Boerrigter, {Michael E.T.I.} and Erik Mullaart and Frits Berends and Jan Vijg",

note = "Funding Information: We thank Dr G.Douglas for critically reading the mansucript and Mr M.Boermans and Mr R.van Boven for preparing the figures. This work was supported by grants from Senetek p.l.c, Sandoz Foundation for Gerontological Research and the Dutch Ministry of Welfare, Health and Cultural Affairs.",

year = "1991",

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doi = "10.1093/carcin/12.1.77",

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T1 - Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea

AU - Boerrigter, Michael E.T.I.

AU - Mullaart, Erik

AU - Berends, Frits

AU - Vijg, Jan

N1 - Funding Information: We thank Dr G.Douglas for critically reading the mansucript and Mr M.Boermans and Mr R.van Boven for preparing the figures. This work was supported by grants from Senetek p.l.c, Sandoz Foundation for Gerontological Research and the Dutch Ministry of Welfare, Health and Cultural Affairs.

PY - 1991/1

Y1 - 1991/1

N2 - We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-Nnitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16% (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h posttreatment no further disappearance was observed. Inter individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3% (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [3H]- thymidine. The cytosine analog 1-β-D-arabinofuranosylcytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.

AB - We investigated the induction and disappearance of DNA lesions that are detected as single-strand breaks (SSBs) with the alkaline filter elution technique, in human peripheral blood lymphocytes (PBLs) following exposure to N-ethyl-Nnitrosourea (ENU). In PBLs of the 15 individuals studied, 35 ± 16% (mean ± SD) of the SSBs present at the end of a 20 min treatment disappeared within 1 h; up to 24 h posttreatment no further disappearance was observed. Inter individual differences in SSB disappearance were considerable; in two cases, almost no SSBs disappeared over the 1 h period. In PBLs of the same 15 individuals 85 ± 3% (mean ± SD) of the SSBs induced by 4 Gy of γ-rays were found to disappear within 1 h, which indicated no interindividual differences. Multiple blood sampling at 1 month intervals indicated that interindividual differences in ENU-induced SSB repair are constant and do not vary with time. The low rate of SSB disappearance appeared to correlate with low cell survival after ENU exposure, measured as a reduction in phytohemagglutinin-stimulated incorporation of [3H]- thymidine. The cytosine analog 1-β-D-arabinofuranosylcytosine completely inhibited SSB disappearance, indicating that the removal of the repairable ENU-induced SSBs involves excision repair events.

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Induction and disappearance of DNA strand breaks and/or alkalilabile sites in human lymphocytes exposed to N-ethyl-N-nitrosourea

Abstract

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