Abstract
Using the alkaline filter elution technique we monitored the induction and disappearance of DNA single-strand breaks (SSB) in 3 different human lymphocyte populations: (1) freshly isolated peripheral blood lymphocytes (PBL); (2) B and T cell-enriched lymphocyte fractions; and (3) actively proliferating T cells, after exposure to ethylnitrosourea (ENU). Between these different lymphocyte populations no significant differences were observed in the number of SSB induced by a 20-min treatment with 0.5 mM ENU. SSB disappearance was observed in PBL of some but not all individuals, confirming our earlier results (Boerrigter et al., 1990a). Determinations on B and T cell-enriched lymphocyte populations indicated that ENU-induced SSB were removed only in T lymphocytes; no significant amount of SSB disappearance was observed in B lymphocytes. In contrast, no differences in SSB repair between B and T lymphocytes were found γ-irradiation. Induction and disappearance of ENU-induced SSB were found not to be dependent on the proliferative status of T lymphocytes; no differences were observed between quiescent PBL or T lymphocytes and actively proliferating T cells from the same donor, with respect to either the rate or the total amount of ENU-induced SSB disappearance.
Original language | English (US) |
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Pages (from-to) | 49-55 |
Number of pages | 7 |
Journal | Mutation Research-DNA Repair |
Volume | 255 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1991 |
Externally published | Yes |
Keywords
- Alkaline filter elution
- B and T lymphocytes
- DNA repair
- Ethylnitrosourea
- human
ASJC Scopus subject areas
- Molecular Biology
- Toxicology
- Genetics