Inducing and measuring apoptotic cell death in mouse pancreatic β-cells and in isolated islets

Wendy M. McKimpson, Richard N. Kitsis

Research output: Contribution to journalArticlepeer-review

Abstract

Type 2 diabetes is mediated by insulin resistance and pancreatic β-cell failure, the latter reflecting a combination of β-cell dysfunction, dedifferentiation, and apoptosis. Quantification of β-cell apoptosis in diabetes can be challenging both with respect to methodology and selection of clinically relevant inducers and readouts. This protocol describes approaches to measure cell death in immortalized β-cells, primary mouse islet preparations, and pancreatic tissue. The resulting information may be useful for mechanistic studies and assessment of the contribution of β-cell death to pathogenesis. For complete details on the use and execution of this protocol, please refer to McKimpson et al. (2021).

Original languageEnglish (US)
Article number101287
JournalSTAR Protocols
Volume3
Issue number2
DOIs
StatePublished - Jun 17 2022

Keywords

  • Cell Biology
  • Cell isolation
  • Cell-based Assays
  • Metabolism

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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