Induced pluripotent stem cells derived from human amnion in chemically defined conditions

Jaroslav Slamecka, Steven McClellan, Anna Wilk, Javier Laurini, Elizabeth Manci, Simon P. Hoerstrup, Benedikt Weber, Laurie Owen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Fetal stem cells are a unique type of adult stem cells that have been suggested to be broadly multipotent with some features of pluripotency. Their clinical potential has been documented but their upgrade to full pluripotency could open up a wide range of cell-based therapies particularly suited for pediatric tissue engineering, longitudinal studies or disease modeling. Here we describe episomal reprogramming of mesenchymal stem cells from the human amnion to pluripotency (AM-iPSC) in chemically defined conditions. The AM-iPSC expressed markers of embryonic stem cells, readily formed teratomas with tissues of all three germ layers present and had a normal karyotype after around 40 passages in culture. We employed novel computational methods to determine the degree of pluripotency from microarray and RNA sequencing data in these novel lines alongside an iPSC and ESC control and found that all lines were deemed pluripotent, however, with variable scores. Differential expression analysis then identified several groups of genes that potentially regulate this variability in lines within the boundaries of pluripotency, including metallothionein proteins. By further studying this variability, characteristics relevant to cell-based therapies, like differentiation propensity, could be uncovered and predicted in the pluripotent stage.

Original languageEnglish (US)
Pages (from-to)330-347
Number of pages18
JournalCell Cycle
Volume17
Issue number3
DOIs
StatePublished - Feb 1 2018
Externally publishedYes

Keywords

  • amnion
  • CellNet
  • episomal reprogramming
  • iPSC
  • microarray
  • PluriTest
  • RNA sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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