Induced Plasma expander-like properties as a function of PEG-chains on extension arm facilitated PEGylation of albumin

"mushroom to Brush-Like" conformational transition of the PEG-albumin conjugate

Ranjit K. Sahu, Parimala Nacharaju, Belur N. Manjula, Seetharama A. Acharya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Plasma expander-like properties of albumin induced on hexa as well as dodecacPEGylation using Extension Arm Facilitated PEGylation platform make it an excellent resuscitation fluid. PEGylation induced changes in the structure, drug binding, and plasma expander-like properties of bovine serum albumin has been now investigated as a function of PEGylation. The molecular volume of albumin increases on PEGylation nearly linearly; in the beginning up to about six PEG chains are conjugated, then plateau off, while the viscosity and colloidal osmotic pressure change very little initially and then increase exponentially as a function of PEG chains conjugated. PEGylation has essentially no influence on the secondary structure or drug properties of albumin. Tryphtophyl fluorescence of albumin is quenched on PEGylation as a direct correlate of the changes in molecular radius of PEG-albumin. It is concluded that hexaPEGylated and dodecaPEGylated albumin belong to two different configurational states of PEG-albumin in terms of packing of PEG-chains on the molecular surface of the protein. The results suggest a transition of PEGylated albumin from the initial mushroom-like conformation to brush conformation as the PEGylation increases. The therapeutic efficacy of the two PEGylated species is needed to establish the optimum level of PEGylation to function as resuscitation fluids.

Original languageEnglish (US)
Pages (from-to)245-256
Number of pages12
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume37
Issue number6
DOIs
StatePublished - 2009

Fingerprint

Agaricales
Brushes
Polyethylene glycols
Albumins
Plasmas
Resuscitation
Conformations
Fluids
Fluorescence
Viscosity
Osmotic Pressure
Proteins
Bovine Serum Albumin
Pharmaceutical Preparations
Membrane Proteins

Keywords

  • Capillary leak
  • Hypovolemic conditions
  • Maleimide-PEG
  • PEGylated albumin
  • Plasma expander

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology

Cite this

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title = "Induced Plasma expander-like properties as a function of PEG-chains on extension arm facilitated PEGylation of albumin: {"}mushroom to Brush-Like{"} conformational transition of the PEG-albumin conjugate",
abstract = "Plasma expander-like properties of albumin induced on hexa as well as dodecacPEGylation using Extension Arm Facilitated PEGylation platform make it an excellent resuscitation fluid. PEGylation induced changes in the structure, drug binding, and plasma expander-like properties of bovine serum albumin has been now investigated as a function of PEGylation. The molecular volume of albumin increases on PEGylation nearly linearly; in the beginning up to about six PEG chains are conjugated, then plateau off, while the viscosity and colloidal osmotic pressure change very little initially and then increase exponentially as a function of PEG chains conjugated. PEGylation has essentially no influence on the secondary structure or drug properties of albumin. Tryphtophyl fluorescence of albumin is quenched on PEGylation as a direct correlate of the changes in molecular radius of PEG-albumin. It is concluded that hexaPEGylated and dodecaPEGylated albumin belong to two different configurational states of PEG-albumin in terms of packing of PEG-chains on the molecular surface of the protein. The results suggest a transition of PEGylated albumin from the initial mushroom-like conformation to brush conformation as the PEGylation increases. The therapeutic efficacy of the two PEGylated species is needed to establish the optimum level of PEGylation to function as resuscitation fluids.",
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T1 - Induced Plasma expander-like properties as a function of PEG-chains on extension arm facilitated PEGylation of albumin

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AU - Nacharaju, Parimala

AU - Manjula, Belur N.

AU - Acharya, Seetharama A.

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N2 - Plasma expander-like properties of albumin induced on hexa as well as dodecacPEGylation using Extension Arm Facilitated PEGylation platform make it an excellent resuscitation fluid. PEGylation induced changes in the structure, drug binding, and plasma expander-like properties of bovine serum albumin has been now investigated as a function of PEGylation. The molecular volume of albumin increases on PEGylation nearly linearly; in the beginning up to about six PEG chains are conjugated, then plateau off, while the viscosity and colloidal osmotic pressure change very little initially and then increase exponentially as a function of PEG chains conjugated. PEGylation has essentially no influence on the secondary structure or drug properties of albumin. Tryphtophyl fluorescence of albumin is quenched on PEGylation as a direct correlate of the changes in molecular radius of PEG-albumin. It is concluded that hexaPEGylated and dodecaPEGylated albumin belong to two different configurational states of PEG-albumin in terms of packing of PEG-chains on the molecular surface of the protein. The results suggest a transition of PEGylated albumin from the initial mushroom-like conformation to brush conformation as the PEGylation increases. The therapeutic efficacy of the two PEGylated species is needed to establish the optimum level of PEGylation to function as resuscitation fluids.

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