Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy

Kinshuk Mitra, James Melvin, Shufang Chang, Kyoungjin Park, Alper Yilmaz, W. Scott Melvin, Ronald X. Xu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We encapsulate indocyanine green (ICG) in poly[(D,L-lactide-co-glycolide)- co-PEG] diblock (PLGA-PEG) microballoons for real-time fluorescence and hyperspectral imaging of biliary anatomy. ICG-loaded microballoons show superior fluorescence characteristics and slower degradation in comparison with pure ICG. The use of ICG-loaded microballoons in biliary imaging is demonstrated in both biliary-simulating phantoms and an ex vivo tissue model. The biliary-simulating phantoms are prepared by embedding ICG-loaded microballoons in agar gel and imaged by a fluorescence imaging module in a Da Vinci surgical robot. The ex vivo model consists of liver, gallbladder, common bile duct, and part of the duodenum freshly dissected from a domestic swine. After ICG-loaded microballoons are injected into the gallbladder, the biliary structure is imaged by both hyperspectral and fluorescence imaging modalities. Advanced spectral analysis and image processing algorithms are developed to classify the tissue types and identify the biliary anatomy. While fluorescence imaging provides dynamic information of movement and flow in the surgical region of interest, data from hyperspectral imaging allow for rapid identification of the bile duct and safe exclusion of any contaminant fluorescence from tissue not part of the biliary anatomy. Our experiments demonstrate the technical feasibility of using ICG-loaded microballoons for biliary imaging in cholecystectomy.

Original languageEnglish (US)
Article number116025
JournalJournal of Biomedical Optics
Volume17
Issue number11
DOIs
StatePublished - Nov 2012
Externally publishedYes

Fingerprint

microballoons
Indocyanine Green
Fluorescence
Imaging techniques
fluorescence
anatomy
Tissue
ducts
Ducts
Polyethylene glycols
swine
exclusion
robots
liver
Liver
Spectrum analysis
embedding
spectrum analysis
contaminants
image processing

Keywords

  • bile duct injury
  • cholecystectomy
  • fluorescence imaging
  • hyperspectral imaging
  • indocyanine green
  • microballoon
  • microbubble
  • microcapsule

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy. / Mitra, Kinshuk; Melvin, James; Chang, Shufang; Park, Kyoungjin; Yilmaz, Alper; Melvin, W. Scott; Xu, Ronald X.

In: Journal of Biomedical Optics, Vol. 17, No. 11, 116025, 11.2012.

Research output: Contribution to journalArticle

Mitra, Kinshuk ; Melvin, James ; Chang, Shufang ; Park, Kyoungjin ; Yilmaz, Alper ; Melvin, W. Scott ; Xu, Ronald X. / Indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 11.
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