Indirect measurements of differential gene expression with cDNA microarrays

Thomas J. Belbin, John Gaspar, Missak Haigentz, Roman Perez-Soler, Steven M. Keller, Michael B. Prystowsky, Geoffrey Childs, Nicholas D. Socci

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The use of universal RNA reference sets is an increasingly common approach to molecular classification studies with cDNA microarrays. Here we evaluated the reliability of indirect measurements of fluorescence ratios with a common RNA reference as a means of identifying differentially expressed genes. Comparisons of direct and indirect measures of differential gene expression showed a strong overall correlation in fluorescence ratio measurements but also a high degree of false positives in our indirect measurements. These results indicated that the application of more stringent ratio filters may be required when assessing differential gene expression utilizing a common RNA reference in classification studies.

Original languageEnglish (US)
Pages (from-to)310-314
Number of pages5
JournalBioTechniques
Volume36
Issue number2
StatePublished - Feb 1 2004

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Belbin, T. J., Gaspar, J., Haigentz, M., Perez-Soler, R., Keller, S. M., Prystowsky, M. B., Childs, G., & Socci, N. D. (2004). Indirect measurements of differential gene expression with cDNA microarrays. BioTechniques, 36(2), 310-314.