Independent immunoglobulin class-switch events occurring in a single myeloma cell line

L. A. Eckhardt, B. K. Birshtein

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Abstract

Five γ2a-producing cell lines, all derived from the γ2b-producing mouse myeloma MPC11, were analyzed for changes in gene structure. Also examined was a cell line (IRC9.7.1) that acts as an intermediate to some of these class switches. All six of the MPC11 variants have undergone DNA rearrangement. Rearrangement sites in the γ2a-producing cells are different in each case and generally do not involve tandem repeat sequences. An enhancer for heavy chain gene transcription was deleted in at least one of these cell lines as a result of its class-switch rearrangement. DNA sequence analysis of cloned genes from two of the MPC11 variants revealed shared sequences at their rearrangement breakpoints. The same sequences are found at the breakpoints of two additional immunoglobulin gene rearrangements in MPC11.

Original languageEnglish (US)
Pages (from-to)856-868
Number of pages13
JournalMolecular and cellular biology
Volume5
Issue number4
DOIs
Publication statusPublished - Jan 1 1985

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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