Independent immunoglobulin class-switch events occurring in a single myeloma cell line

L. A. Eckhardt, B. K. Birshtein

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Five γ2a-producing cell lines, all derived from the γ2b-producing mouse myeloma MPC11, were analyzed for changes in gene structure. Also examined was a cell line (IRC9.7.1) that acts as an intermediate to some of these class switches. All six of the MPC11 variants have undergone DNA rearrangement. Rearrangement sites in the γ2a-producing cells are different in each case and generally do not involve tandem repeat sequences. An enhancer for heavy chain gene transcription was deleted in at least one of these cell lines as a result of its class-switch rearrangement. DNA sequence analysis of cloned genes from two of the MPC11 variants revealed shared sequences at their rearrangement breakpoints. The same sequences are found at the breakpoints of two additional immunoglobulin gene rearrangements in MPC11.

Original languageEnglish (US)
Pages (from-to)856-868
Number of pages13
JournalMolecular and Cellular Biology
Volume5
Issue number4
StatePublished - 1985

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Immunoglobulin Isotypes
Gene Rearrangement
Cell Line
Genes
Immunoglobulin Genes
Tandem Repeat Sequences
DNA Sequence Analysis

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Independent immunoglobulin class-switch events occurring in a single myeloma cell line. / Eckhardt, L. A.; Birshtein, B. K.

In: Molecular and Cellular Biology, Vol. 5, No. 4, 1985, p. 856-868.

Research output: Contribution to journalArticle

Eckhardt, L. A. ; Birshtein, B. K. / Independent immunoglobulin class-switch events occurring in a single myeloma cell line. In: Molecular and Cellular Biology. 1985 ; Vol. 5, No. 4. pp. 856-868.
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