Increased vascular sensitivity and connexin43 expression after sympathetic denervation

David P. Slovut, Shyamal H. Mehta, Anne M. Dorrance, Frank C. Brosius, Stephanie W. Watts, R. Clinton Webb

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: Following denervation, arteries demonstrate a heightened sensitivity to α-adrenergic agonists and increased oscillatory contractions that may partly result from increased gap junction expression. Hence, we wanted to study the effect of sympathetic denervation on connexin43 (Cx43) expression and agonist-induced contractility in the vascular smooth muscle (VSM). Methods: Effects of denervation with reserpine (3 mg/kg/day, i.p.) or topical 5% phenol-glycerol on VSM contractions and expression of the gap junction Cx43 mRNA by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting for Cx43 protein were examined. Wistar-Kyoto (WKY) rat tail arteries were exposed to norepinephrine (NE) (10-9-10-5 M). Reactivity was also examined in the carotid arteries and thoracic aortas from Cx43 heterozygote deficient (KO) mice. Results: The concentration for NE-induced contraction was lower in reserpine- and phenol-treated vessels than controls (p<0.05). NE-induced oscillatory activity (OA) was seen in 5/5 reserpine- and 5/8 phenol-treated vessels vs. 0/12 controls (p<0.05). Spontaneous OA was observed more frequently in carotid and aortic rings from WT than Cx43 KO rings. Cumulative OA in response to α-adrenergic stimulation was significantly greater in WT carotid (429±101 vs. 128±7 mN s, p<0.05) and aortic rings (337±85 vs. 134±11 mN s, p<0.05) than in Cx43 KO rings. Following denervation, RT-PCR showed significantly increased levels of Cx43 mRNA (p<0.05). Western blot analysis revealed near doubling of Cx43 protein (p<0.05). Conclusion: We conclude that sympathetic denervation results in increased expression of Cx43, which in turn, contributes to increased spontaneous and agonist-induced OA in VSM.

Original languageEnglish (US)
Pages (from-to)388-396
Number of pages9
JournalCardiovascular Research
Volume62
Issue number2
DOIs
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Connexin 43
Sympathectomy
Blood Vessels
Reserpine
Denervation
Phenol
Vascular Smooth Muscle
Norepinephrine
Gap Junctions
Reverse Transcriptase Polymerase Chain Reaction
Arteries
Western Blotting
Adrenergic Agonists
Messenger RNA
Inbred WKY Rats
Heterozygote
Muscle Contraction
Thoracic Aorta
Carotid Arteries
Adrenergic Agents

Keywords

  • Connexin43
  • Gap junctions
  • Sympathetic denervation
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Slovut, D. P., Mehta, S. H., Dorrance, A. M., Brosius, F. C., Watts, S. W., & Webb, R. C. (2004). Increased vascular sensitivity and connexin43 expression after sympathetic denervation. Cardiovascular Research, 62(2), 388-396. https://doi.org/10.1016/j.cardiores.2003.12.024

Increased vascular sensitivity and connexin43 expression after sympathetic denervation. / Slovut, David P.; Mehta, Shyamal H.; Dorrance, Anne M.; Brosius, Frank C.; Watts, Stephanie W.; Webb, R. Clinton.

In: Cardiovascular Research, Vol. 62, No. 2, 01.05.2004, p. 388-396.

Research output: Contribution to journalArticle

Slovut, DP, Mehta, SH, Dorrance, AM, Brosius, FC, Watts, SW & Webb, RC 2004, 'Increased vascular sensitivity and connexin43 expression after sympathetic denervation', Cardiovascular Research, vol. 62, no. 2, pp. 388-396. https://doi.org/10.1016/j.cardiores.2003.12.024
Slovut, David P. ; Mehta, Shyamal H. ; Dorrance, Anne M. ; Brosius, Frank C. ; Watts, Stephanie W. ; Webb, R. Clinton. / Increased vascular sensitivity and connexin43 expression after sympathetic denervation. In: Cardiovascular Research. 2004 ; Vol. 62, No. 2. pp. 388-396.
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AU - Webb, R. Clinton

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