Increased susceptibility of brain slices from carbonic anhydrase II-deficient mice to low [Mg2+]0-induced seizures

Libor Velíšek, Solomon L. Moshé, Patrie K. Stanton

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Brain pH is thought to be an influential factor in determining susceptibility to seizures. We compared the susceptibility of brain slices from carbonic anhydrase II (CA II)-deficient mice to epileptiform activity induced by low extracellular [Mg2+], with slices from normal littermates, both bathed in artificial cerebrospinal fluid at pH 7.3. In both entorhinal cortex and hippocampal field CA1, epileptiform activity started earlier in CA II-deficient slices. Raising extracellular [CO2] (20%; extracellular pH, 6.7) reversibly blocked the epileptiform activity in normal, but not in CA II-deficient, slices. The data, combined with previous in vivo findings showing an increased resistance of mutants to seizures, suggest the presence of in vivo anticonvulsant acidosis with long-term compensatory changes that lead to in vitro 'proconvulsant' behavior in CA II-deficient slices clamped at pH 7.3.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalNeuroscience Letters
Volume207
Issue number3
DOIs
StatePublished - Apr 5 1996

Keywords

  • Acidosis
  • Carbonic anhydrase
  • Entorhinal cortex
  • Epilepsy
  • Hippocampus
  • N-methyl-D-aspartate receptors

ASJC Scopus subject areas

  • Neuroscience(all)

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