Increased muscle fatigability in GLUT-4-deficient mice

M. Gorselink, M. R. Drost, K. F.J. De Brouwer, G. Schaart, G. P.J. Van Kranenburg, T. H.M. Roemen, M. Van Bilsen, M. J. Charron, G. J. Van Der Vusse

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

GLUT-4 plays a predominant role in glucose uptake during muscle contraction. In the present study, we have investigated in mice whether disruption of the GLUT-4 gene affects isometric and shortening contractile performance of the dorsal flexor muscle complex in situ. Moreover, we have explored the hypothesis that lack of GLUT-4 enhances muscle fatigability. Isometric performance normalized to muscle mass during a single tetanic contraction did not differ between wild-type (WT) and GLUT-4 deficient [GLUT-4(-/-)] mice. Shortening contractions, however, revealed a significant 1.4-fold decrease in peak power per unit mass, most likely caused by the fiber-type transition from fast-glycolytic fibers (IIB) to fast-oxidative fibers (IIA) in GLUT-4(-/-) dorsal flexors. In addition, the resting glycogen content was significantly lower (34%) in the dorsal flexor complex of GLUT-4(-/-) mice than in WT mice. Moreover, the muscle complex of GLUT-4(-/-) mice showed enhanced susceptibility to fatigue, which may be related to the decline in the muscle carbohydrate store. The significant decrease in relative work output during the steady-state phase of the fatigue protocol suggests that energy supply via alternative routes is not capable to compensate fully for the lack of GLUT-4.

Original languageEnglish (US)
Pages (from-to)E348-E354
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume282
Issue number2 45-2
DOIs
StatePublished - Jan 1 2002

Keywords

  • Electrical stimulation
  • Skeletal muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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    Gorselink, M., Drost, M. R., De Brouwer, K. F. J., Schaart, G., Van Kranenburg, G. P. J., Roemen, T. H. M., Van Bilsen, M., Charron, M. J., & Van Der Vusse, G. J. (2002). Increased muscle fatigability in GLUT-4-deficient mice. American Journal of Physiology - Endocrinology and Metabolism, 282(2 45-2), E348-E354. https://doi.org/10.1152/ajpendo.00085.2001