The propensity of site-specific carboxymethylation and Propylation of Val-1(α) of Hb to attenuate the reductive hexaPEGylation-induced dissociation of tetramers has been investigated. Only reductive Propylation of Val-1(α), which increases the stability of oxy Hb, attenuates the reductive hexaPEGylation-induced dissociation. Increasing the stability of the oxy conformation of Hb by chemical or genetic approaches is a strategy to generate PEGylated Hbs with native-like tetramer stability using direct PEGylation platforms. This new approach and EAF-PEGylation are the only two alternate PEGylation strategies available to design stable second-generation vasoinactive uncrosslinked PEGylated Hbs with native-like tetramer stability.
|Original language||English (US)|
|Number of pages||10|
|Journal||Artificial Cells, Blood Substitutes, and Biotechnology|
|State||Published - Apr 1 2011|
ASJC Scopus subject areas
- Biomedical Engineering