Increased expression and characterization of two distinct folate binding proteins in murine erythroleukemia cells

Kevin E. Brigle, Michael J. Spinella, Eric H. Westin, I. David Goldman

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We previously identified two membrane-bound folate binding proteins, FBP1 and FBP2, in murine L1210 leukemia cells. We now report on the development of two variant murine erythroleukemia cell lines that were used for direct comparison and biochemical characterization of the two murine folate binding proteins. Based on the results of northern analysis and the mobilities of affinity-labeled proteins on polyacrylamide gels, these cell lines exhibit specific up-regulated expression of FBP1 or FBP2. The affinities of the folate binding proteins for various (anti)folates were determined based upon the ability of the compounds to inhibit binding of [3H]jfolic acid. The two proteins exhibited considerably different affinities and stereospecificities and, in general, FBP2 consistently bound each test compound with lesser affinity than FBP1. Both proteins displayed greatest affinity for folic acid, 5-methyltetrahydrofolate, and the antifolates CB3717 and 5,10-dideazatetrahydrofolate (DDATHF). Conversely, the proteins exhibited poor affinity for the dihydrofolate reductase inhibitors methotrexate and aminopterin. For 5-formyltetrahydrofolate, FBP1 had high affinity for the (6S) diastereoisomer, whereas FBP2 showed preference for the non-physiologic (6R) diastereoisomer. The binding properties of FBP1 and FBP2 overexpressed in these cell lines closely paralleled those of their respective human homologs. These lines provide a model system in which to examine the biochemical characteristics of the individual folate binding proteins without the potential problems associated with expression of proteins in dissimilar cell lines.

Original languageEnglish (US)
Pages (from-to)337-345
Number of pages9
JournalBiochemical Pharmacology
Volume47
Issue number2
DOIs
StatePublished - Jan 20 1994
Externally publishedYes

Fingerprint

Leukemia, Erythroblastic, Acute
Folic Acid
Carrier Proteins
Cells
Folic Acid Antagonists
Cell Line
Proteins
Aminopterin
Leukemia L1210
Leucovorin
Methotrexate
Membranes
Acids

Keywords

  • binding affinity
  • erythroleukemia
  • folate binding protein

ASJC Scopus subject areas

  • Pharmacology

Cite this

Increased expression and characterization of two distinct folate binding proteins in murine erythroleukemia cells. / Brigle, Kevin E.; Spinella, Michael J.; Westin, Eric H.; Goldman, I. David.

In: Biochemical Pharmacology, Vol. 47, No. 2, 20.01.1994, p. 337-345.

Research output: Contribution to journalArticle

@article{f5214d7dc4544324a9e9b0f1509e83da,
title = "Increased expression and characterization of two distinct folate binding proteins in murine erythroleukemia cells",
abstract = "We previously identified two membrane-bound folate binding proteins, FBP1 and FBP2, in murine L1210 leukemia cells. We now report on the development of two variant murine erythroleukemia cell lines that were used for direct comparison and biochemical characterization of the two murine folate binding proteins. Based on the results of northern analysis and the mobilities of affinity-labeled proteins on polyacrylamide gels, these cell lines exhibit specific up-regulated expression of FBP1 or FBP2. The affinities of the folate binding proteins for various (anti)folates were determined based upon the ability of the compounds to inhibit binding of [3H]jfolic acid. The two proteins exhibited considerably different affinities and stereospecificities and, in general, FBP2 consistently bound each test compound with lesser affinity than FBP1. Both proteins displayed greatest affinity for folic acid, 5-methyltetrahydrofolate, and the antifolates CB3717 and 5,10-dideazatetrahydrofolate (DDATHF). Conversely, the proteins exhibited poor affinity for the dihydrofolate reductase inhibitors methotrexate and aminopterin. For 5-formyltetrahydrofolate, FBP1 had high affinity for the (6S) diastereoisomer, whereas FBP2 showed preference for the non-physiologic (6R) diastereoisomer. The binding properties of FBP1 and FBP2 overexpressed in these cell lines closely paralleled those of their respective human homologs. These lines provide a model system in which to examine the biochemical characteristics of the individual folate binding proteins without the potential problems associated with expression of proteins in dissimilar cell lines.",
keywords = "binding affinity, erythroleukemia, folate binding protein",
author = "Brigle, {Kevin E.} and Spinella, {Michael J.} and Westin, {Eric H.} and Goldman, {I. David}",
year = "1994",
month = "1",
day = "20",
doi = "10.1016/0006-2952(94)90025-6",
language = "English (US)",
volume = "47",
pages = "337--345",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Increased expression and characterization of two distinct folate binding proteins in murine erythroleukemia cells

AU - Brigle, Kevin E.

AU - Spinella, Michael J.

AU - Westin, Eric H.

AU - Goldman, I. David

PY - 1994/1/20

Y1 - 1994/1/20

N2 - We previously identified two membrane-bound folate binding proteins, FBP1 and FBP2, in murine L1210 leukemia cells. We now report on the development of two variant murine erythroleukemia cell lines that were used for direct comparison and biochemical characterization of the two murine folate binding proteins. Based on the results of northern analysis and the mobilities of affinity-labeled proteins on polyacrylamide gels, these cell lines exhibit specific up-regulated expression of FBP1 or FBP2. The affinities of the folate binding proteins for various (anti)folates were determined based upon the ability of the compounds to inhibit binding of [3H]jfolic acid. The two proteins exhibited considerably different affinities and stereospecificities and, in general, FBP2 consistently bound each test compound with lesser affinity than FBP1. Both proteins displayed greatest affinity for folic acid, 5-methyltetrahydrofolate, and the antifolates CB3717 and 5,10-dideazatetrahydrofolate (DDATHF). Conversely, the proteins exhibited poor affinity for the dihydrofolate reductase inhibitors methotrexate and aminopterin. For 5-formyltetrahydrofolate, FBP1 had high affinity for the (6S) diastereoisomer, whereas FBP2 showed preference for the non-physiologic (6R) diastereoisomer. The binding properties of FBP1 and FBP2 overexpressed in these cell lines closely paralleled those of their respective human homologs. These lines provide a model system in which to examine the biochemical characteristics of the individual folate binding proteins without the potential problems associated with expression of proteins in dissimilar cell lines.

AB - We previously identified two membrane-bound folate binding proteins, FBP1 and FBP2, in murine L1210 leukemia cells. We now report on the development of two variant murine erythroleukemia cell lines that were used for direct comparison and biochemical characterization of the two murine folate binding proteins. Based on the results of northern analysis and the mobilities of affinity-labeled proteins on polyacrylamide gels, these cell lines exhibit specific up-regulated expression of FBP1 or FBP2. The affinities of the folate binding proteins for various (anti)folates were determined based upon the ability of the compounds to inhibit binding of [3H]jfolic acid. The two proteins exhibited considerably different affinities and stereospecificities and, in general, FBP2 consistently bound each test compound with lesser affinity than FBP1. Both proteins displayed greatest affinity for folic acid, 5-methyltetrahydrofolate, and the antifolates CB3717 and 5,10-dideazatetrahydrofolate (DDATHF). Conversely, the proteins exhibited poor affinity for the dihydrofolate reductase inhibitors methotrexate and aminopterin. For 5-formyltetrahydrofolate, FBP1 had high affinity for the (6S) diastereoisomer, whereas FBP2 showed preference for the non-physiologic (6R) diastereoisomer. The binding properties of FBP1 and FBP2 overexpressed in these cell lines closely paralleled those of their respective human homologs. These lines provide a model system in which to examine the biochemical characteristics of the individual folate binding proteins without the potential problems associated with expression of proteins in dissimilar cell lines.

KW - binding affinity

KW - erythroleukemia

KW - folate binding protein

UR - http://www.scopus.com/inward/record.url?scp=0028008481&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028008481&partnerID=8YFLogxK

U2 - 10.1016/0006-2952(94)90025-6

DO - 10.1016/0006-2952(94)90025-6

M3 - Article

VL - 47

SP - 337

EP - 345

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 2

ER -