Increased epinephrine and skeletal muscle responses to hypoglycemia in non-insulin-dependent diabetes mellitus

Harry Shamoon, Seth Friedman, Carlos Canton, Leon Zacharowicz, Meizhu Hu, Luciano Rossetti

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

We evaluated skeletal muscle counterregulation during hypoglycemia in nine subjects with non-insulin-dependent diabetes mellitus (NIDDM) (HbA(1c) 9.4±0.5%, nl < 6.2%) compared with six normal controls, matched for age (51±3 and 49±5 yr, respectively) and body mass index (27.3±1.2 and 27.0±2.1 kg/m2). After 60 min of euglycemia (plasma insulin ~ 140 μU/ml), plasma glucose was lowered to 62±2 mg/dl by 120 min. Hypoglycemia induced a 2.2-fold greater increase in plasma epinephrine in NIDDM (P < 0.001), while the plasma glucagon response was blunted (P < 0.01). Hepatic glucose output ([3H-3]glucose) suppressed similarly during euglycemia, but during hypoglycemia was greater in NIDDM (P < 0.005). Conversely, glucose uptake during euglycemia was 150% greater in controls (P < 0.01) and remained persistently higher than in NIDDM during hypoglycemia. In NIDDM, plasma FFA concentrations were approximately fivefold greater (P < 0.001), and plasma lactate levels were ~ 40% higher than in controls during hypoglycemia (P < 0.01); the rates of glycolysis from plasma glucose were similar in the two groups despite a 49% lower rate of glucose uptake in NIDDM (3.4±0.9 vs. 6.9±1.3 mg/kg per minute, P < 0.001). Muscle glycogen synthase activity fell by 42% with hypoglycemia (P < 0.01) in NIDDM but not in controls. In addition, glycogen phosphorylase was activated by 56% during hypoglycemia in NIDDM only (P < 0.01). Muscle glucose-6-phosphate concentrations rose during hypoglycemia by a twofold greater increment in NIDDM (P < 0.01). Thus, skeletal muscle participates in hypoglycemia counterregulation in NIDDM, directly by decreased removal of plasma glucose and, indirectly, by providing lactate for hepatic gluconeogenesis. Consequently, in addition to inherent insulin resistance in NIDDM, the enhanced plasma epinephrine response during hypoglycemia may partially offset impaired glucagon secretion and counteract the effects of hyperinsulinemia on liver, fat, and skeletal muscle.

Original languageEnglish (US)
Pages (from-to)2562-2571
Number of pages10
JournalJournal of Clinical Investigation
Volume93
Issue number6
DOIs
StatePublished - Jun 1994

Keywords

  • NIDDM
  • counte rregulation
  • epinephrine
  • glucagon
  • glucose-6- phosphate
  • glycogen
  • glycogen phosphorylase
  • glycogen synthase
  • hepatic glucose output
  • humans
  • hypoglycemia
  • insulin
  • muscle
  • substrates

ASJC Scopus subject areas

  • Medicine(all)

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