Increase in hepatic mitochondrial α-glycerophosphate dehydrogenase activity after surgical stress in hyperthyroid rats

Y. Khawaja, H. Dobnig, L. E. Shapiro, Martin I. Surks

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Abstract

We have established a rat model to investigate the relationship among serum thyroid hormones, nuclear iodothyronine receptors, and biological responses in thyrotoxic and euthyroid rats after surgical stress. Euthyroid or hyperthyroid rate (1.0 Mg T3/ml drinking water for 14 days) were subjected to surgical stress (ether anesthesia, laparotomy plus 50 mg talc, ip). Groups of control or stressed rats were killed 1, 2, and 3 days after surgical stress for measurement of thyroid hormone-responsive hepatic enzymes, α-glycerophosphate dehydrogenase (αGPD) and cytosol malic enzyme, serum T3, T3 nuclear receptors, and GH mRNA. Thyrotoxic rats had a 3.8-fold increase in αGPD compared to euthyroid rats before surgical stress; αGPD increased further to 5.9-fold the euthyroid value 1 day after surgery (P < 0.001) to 5.1-fold after 2 days (P < 0.05) and was similar to control after 3 days. Malic enzyme activity increased 10.5-fold before surgical stress and decreased slightly after surgical stress perhaps due to multifactorial regulation of that enzyme. No increases in T3 nuclear receptor or GH mRNA occurred after surgery in hyperthyroid rats or in any of the above parameters after surgical stress in euthyroid rats. Our findings suggest that increased αGPD after surgical stress in thyrotoxic rats was not due to either increased serum total T3 or free T3 or to increased T3-nuclear receptor complexes. Increased αGPD, therefore, appeared to be a consequence of postreceptor amplification of this thyroidal response.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalEndocrinology
Volume127
Issue number1
StatePublished - Jul 1990

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Glycerolphosphate Dehydrogenase
Hyperthyroidism
Liver
Cytoplasmic and Nuclear Receptors
Thyroid Hormone Receptors
Enzymes
Thyroid Hormones
Serum
Talc
Messenger RNA
Ambulatory Surgical Procedures
Drinking Water
Ether
Cytosol
Laparotomy
Anesthesia
Control Groups

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Increase in hepatic mitochondrial α-glycerophosphate dehydrogenase activity after surgical stress in hyperthyroid rats. / Khawaja, Y.; Dobnig, H.; Shapiro, L. E.; Surks, Martin I.

In: Endocrinology, Vol. 127, No. 1, 07.1990, p. 387-393.

Research output: Contribution to journalArticle

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