Inactivation of chicken mitochondrial phosphoenolpyruvate carboxykinase by o-phthalaldehyde

Cheau Yun Chen, Frances A. Emig, Vern L. Schramm, David E. Ash

Research output: Contribution to journalArticle

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Abstract

Chicken liver mitochondrial phosphoenolpyruvate carboxykinase is inactivated by o-phthalaldehyde. The inactivation followed pseudo first-order kinetics, and the second-order rate constant for the inactivation process was 29 M-1 s-1 at pH 7.5 and 25 °C. The modified enzyme showed maximal fluorescence at 427 nm upon excitation at 337 nm, consistent with the formation of isoindole derivatives by the cross-linking of proximal cysteine and lysine residues. Activities in the physiologic reaction and in the oxaloacetate decarboxylase reaction were lost in parallel upon modification with o-phthalaldehyde. Plots of (percent of residual activity) versus (mol of isoindole incorporated/mol of enzyme) were biphasic, with the initial loss of enzymatic activity corresponding to the incorporation of one isoindole derivative/enzyme molecule. Complete inactivation of the enzyme was accompanied by the incorporation of 3 mol of isoindole/mol of enzyme, βSulfopyruvate, an isoelectronic analogue of oxaloacetate, completely protected the enzyme from reacting with o-phthalaldehyde. Other substrates provided protection from inactivation, in decreasing order of protection: oxaloacetate > phosphoenolpyruvate > MgGDP, MgGTP > oxalate. cysteine 31 and lysine 39 have been identified as the rapidly reacting pair in isoindole formation and enzyme inactivation. Lysine M and cysteine 60 are also involved in isoindole formation in the completely inactivated enzyme. These reactive cysteine residues do not correspond to the reactive cysteine residue identified in previous iodoacetate labeling studies with the chicken mitochondrial enzyme (Makinen, A. L., and Nowak, T. (1989) J. Biol. Chem. 264, 12148-12157). Protection experiments suggest that the sites of o-phthalaldehyde modification become inaccessible when the oxaloacetate/phosphoenolpyruvate binding site is saturated, and sequence analyses indicate that cysteine 31 is located in the putative phosphoenolpyruvate binding site.

Original languageEnglish (US)
Pages (from-to)16645-16652
Number of pages8
JournalJournal of Biological Chemistry
Volume266
Issue number25
StatePublished - 1991

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o-Phthalaldehyde
Phosphoenolpyruvate
Isoindoles
Chickens
Cysteine
Enzymes
Oxaloacetic Acid
Lysine
oxaloacetate decarboxylase
Binding Sites
Iodoacetates
Derivatives
Oxalates
Liver
Labeling
Sequence Analysis
Rate constants
Fluorescence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inactivation of chicken mitochondrial phosphoenolpyruvate carboxykinase by o-phthalaldehyde. / Chen, Cheau Yun; Emig, Frances A.; Schramm, Vern L.; Ash, David E.

In: Journal of Biological Chemistry, Vol. 266, No. 25, 1991, p. 16645-16652.

Research output: Contribution to journalArticle

Chen, Cheau Yun ; Emig, Frances A. ; Schramm, Vern L. ; Ash, David E. / Inactivation of chicken mitochondrial phosphoenolpyruvate carboxykinase by o-phthalaldehyde. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 25. pp. 16645-16652.
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