TY - JOUR
T1 - In vivo transcriptome of plasmodium falciparum reveals overexpression of transcripts that encode surface proteins
AU - Daily, Johanna P.
AU - Le Roch, Karine G.
AU - Sarr, Ousmane
AU - Ndiaye, Oaouda
AU - Lukens, Amanda
AU - Zhou, Yingyao
AU - Ndir, Omar
AU - Mboup, Soulyemane
AU - Sultan, Ali
AU - Winzeler, Elizabeth A.
AU - Wirth, Dyann F.
N1 - Funding Information:
Received 14 June 2004; accepted 20 October 2004; electronically published 28 February 2005. Financial support: National Institute of Allergy and Infectious Diseases (5K23AI054518 to J.P.D.); Fogarty International Training Grant (5D43TW001503 to D.F.W.); Ellison Medical Research Foundation (New Scholars Award to E.A.W.). Reprints or correspondence: Dr. Johanna P. Daily, Harvard School of Public Health, 665 Huntington Ave., Building One, 1st Fl., Boston, MA 02115 (jdaily@partners.org).
PY - 2005/4/1
Y1 - 2005/4/1
N2 - Infections with the human parasite Plasmodium falciparum continue to present a great challenge to global health, Fundamental questions regarding the molecular basis of virulence and immune evasion in P. falciparum have been only partially answered. Because of the parasite's intracellular location and complex life cycle, standard genetic approaches to the study of the pathogenesis of malaria have been limited. The present study presents a novel approach to the identification of the biological processes involved in host-pathogen interactions, one that is based on the analysis of in vivo P. falciparum transcripts. We demonstrate that a sufficient quantity of P. falciparum RNA transcripts can be derived from a small blood sample from infected patients for whole-genome microarray analysis. Overall, excellent correlation was observed between the transcriptomes derived from in vivo samples and in vitro samples with ring-stage P. falciparum 3D7 reference strain. However, gene families that encode surface proteins are overexpressed in vivo. Moreover, this analysis has identified a new family of hypothetical genes that may encode surface variant antigens. Comparative studies of the transcriptomes derived from in vivo samples and in vitro 3D7 samples may identify important strategies used by the pathogen for survival in the human host and highlight, for vaccine development, new candidate antigens that were not previously identified through the use of in vitro cultures.
AB - Infections with the human parasite Plasmodium falciparum continue to present a great challenge to global health, Fundamental questions regarding the molecular basis of virulence and immune evasion in P. falciparum have been only partially answered. Because of the parasite's intracellular location and complex life cycle, standard genetic approaches to the study of the pathogenesis of malaria have been limited. The present study presents a novel approach to the identification of the biological processes involved in host-pathogen interactions, one that is based on the analysis of in vivo P. falciparum transcripts. We demonstrate that a sufficient quantity of P. falciparum RNA transcripts can be derived from a small blood sample from infected patients for whole-genome microarray analysis. Overall, excellent correlation was observed between the transcriptomes derived from in vivo samples and in vitro samples with ring-stage P. falciparum 3D7 reference strain. However, gene families that encode surface proteins are overexpressed in vivo. Moreover, this analysis has identified a new family of hypothetical genes that may encode surface variant antigens. Comparative studies of the transcriptomes derived from in vivo samples and in vitro 3D7 samples may identify important strategies used by the pathogen for survival in the human host and highlight, for vaccine development, new candidate antigens that were not previously identified through the use of in vitro cultures.
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U2 - 10.1086/428289
DO - 10.1086/428289
M3 - Article
C2 - 15747257
AN - SCOPUS:20144378871
SN - 0022-1899
VL - 191
SP - 1196
EP - 1203
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 7
ER -