In vivo proton MR spectroscopy of pancreatic neuroendocrine tumors in a multiple endocrine neoplasia type 1 conditional knockout mouse model

Min Hui Cui, Craig A. Branch, Sean M. Cahill, Thomas J. Quinn, Asha Adem, Steven K. Libutti, Ziqiang Yuan

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Purpose MR spectroscopy (MRS) can improve diagnosis and follow treatment in cancer. However, no study has yet reported application of in vivo 1H-MRS in malignant pancreatic lesions. This study quantitatively determined whether in vivo 1H-MRS on multiple endocrine neoplasia type 1 (Men1) conditional knockout (KO) mice and their wild type (WT) littermates could detect differences in total choline (tCho) levels between tumor and control pancreas. Methods Relative tCho levels in pancreatic tumors or pancreata from KO and WT mice were determined using in vivo 1H-MRS at 9.4 T. The levels of Cho-containing compounds were also quantified using in vitro 1H-NMR on extracts of pancreatic tissues from KO and WT mice, respectively, and on extracts of pancreatic tissues from patients with pancreatic neuroendocrine tumors (PNETs). Results tCho levels measured by in vivo 1H-MRS were significantly higher in PNETs from KO mice compared to the normal pancreas from WT mice. The elevated choline-containing compounds were also identified in pancreatic tumors from KO mice and tissues from patients with PNETs via in vitro 1H-NMR. Conclusion These results indicate the potential use of tCho levels estimated via in vivo 1H-MRS in differentiating malignant pancreatic tumors from benign tumors. Magn Reson Med 74:1221-1226, 2015.

Original languageEnglish (US)
Pages (from-to)1221-1226
Number of pages6
JournalMagnetic Resonance in Medicine
Volume74
Issue number5
DOIs
StatePublished - Nov 2015

Keywords

  • Men1 conditional knockout mouse model
  • in vivo proton MR spectroscopy
  • pancreatic neuroendocrine tumors
  • total choline levels

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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