In vivo photoswitchable flow cytometry for direct tracking of single circulating Tumor Cells

Dmitry A. Nedosekin, Vladislav Verkhusha, Alexander V. Melerzanov, Vladimir P. Zharov, Ekaterina I. Galanzha

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Photoswitchable fluorescent proteins (PSFPs) that change their color in response to light have led to breakthroughs in studying static cells. However, using PSFPs to study cells in dynamic conditions is challenging. Here we introduce a method for in vivo ultrafast photoswitching of PSFPs that provides labeling and tracking of single circulating cells. Using in vivo multicolor flow cytometry, this method demonstrated the capability for studying recirculation, migration, and distribution of circulating tumor cells (CTCs) during metastasis progression. In tumor-bearing mice, it enabled monitoring of real-time dynamics of CTCs released from primary tumor, identifying dormant cells, and imaging of CTCs colonizing a primary tumor (self-seeding) or existing metastasis (reseeding). Integration of genetically encoded PSFPs, fast photoswitching, flow cytometry, and imaging makes in vivo single cell analysis in the circulation feasible to provide insights into the behavior of CTCs and potentially immune-related and bacterial cells in circulation.

Original languageEnglish (US)
Pages (from-to)792-801
Number of pages10
JournalChemistry and Biology
Volume21
Issue number6
DOIs
StatePublished - Jun 19 2014

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Circulating Neoplastic Cells
Flow cytometry
Tumors
Flow Cytometry
Cells
Proteins
Single-Cell Analysis
Neoplasm Metastasis
Neoplasms
Bearings (structural)
Imaging techniques
Color
Labeling
Light
Monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Medicine(all)

Cite this

In vivo photoswitchable flow cytometry for direct tracking of single circulating Tumor Cells. / Nedosekin, Dmitry A.; Verkhusha, Vladislav; Melerzanov, Alexander V.; Zharov, Vladimir P.; Galanzha, Ekaterina I.

In: Chemistry and Biology, Vol. 21, No. 6, 19.06.2014, p. 792-801.

Research output: Contribution to journalArticle

Nedosekin, Dmitry A. ; Verkhusha, Vladislav ; Melerzanov, Alexander V. ; Zharov, Vladimir P. ; Galanzha, Ekaterina I. / In vivo photoswitchable flow cytometry for direct tracking of single circulating Tumor Cells. In: Chemistry and Biology. 2014 ; Vol. 21, No. 6. pp. 792-801.
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