In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes

Lisa Roux; Eran Stark; Lucas Sjulson; György Buzsáki

doi:10.1016/j.conb.2013.12.013

In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes

Lisa Roux, Eran Stark, Lucas Sjulson, György Buzsáki

Research output: Contribution to journal › Review article › peer-review

65 Scopus citations

Abstract

Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.

Original language	English (US)
Pages (from-to)	88-95
Number of pages	8
Journal	Current Opinion in Neurobiology
Volume	26
DOIs	https://doi.org/10.1016/j.conb.2013.12.013
State	Published - Jun 2014
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.conb.2013.12.013

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title = "In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes",

abstract = "Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.",

author = "Lisa Roux and Eran Stark and Lucas Sjulson and Gy{\"o}rgy Buzs{\'a}ki",

note = "Funding Information: We thank S. Royer, A. Berenyi and R. Eichler for their support and E. Schomburg for his comments on the manuscript. Research was funded by National Institute of Health Grants NS34994 , MH54671 and NS074015 , the Human Frontier Science Program and the J.D. McDonnell Foundation . LR received also support from the Bettencourt Schueller Foundation. LS is supported by NCATS grant UL1 TR000038 .",

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doi = "10.1016/j.conb.2013.12.013",

language = "English (US)",

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T1 - In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes

AU - Roux, Lisa

AU - Stark, Eran

AU - Sjulson, Lucas

AU - Buzsáki, György

N1 - Funding Information: We thank S. Royer, A. Berenyi and R. Eichler for their support and E. Schomburg for his comments on the manuscript. Research was funded by National Institute of Health Grants NS34994 , MH54671 and NS074015 , the Human Frontier Science Program and the J.D. McDonnell Foundation . LR received also support from the Bettencourt Schueller Foundation. LS is supported by NCATS grant UL1 TR000038 .

PY - 2014/6

Y1 - 2014/6

N2 - Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.

AB - Identification and manipulation of different GABAergic interneuron classes in the behaving animal are important to understand their role in circuit dynamics and behavior. The combination of optogenetics and large-scale neuronal recordings allows specific interneuron populations to be identified and perturbed for circuit analysis in intact animals. A crucial aspect of this approach is coupling electrophysiological recording with spatially and temporally precise light delivery. Focal multisite illumination of neuronal activators and silencers in predetermined temporal configurations or a closed loop manner opens the door to addressing many novel questions. Recent progress demonstrates the utility and power of this novel technique for interneuron research.

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In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes

Abstract

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