In vivo kinetics of Cajal body components

Miroslav Dundr, Michael D. Hebert, Tatiana S. Karpova, David Stanek, Hongzi Xu, Karl B. Shpargel, U. Thomas Meier, Karla M. Neugebauer, A. Gregory Matera, Tom Misteli

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Cajal bodies (CBs) are subnuclear domains implicated in small nuclear ribonucleoprotein (snRNP) biogenesis. In most cell types, CBs coincide with nuclear gems, which contain the survival of motor neurons (SMN) complex, an essential snRNP assembly factor. Here, we analyze the exchange kinetics of multiple components of CBs and gems in living cells using photobleaching microscopy. We demonstrate differences in dissociation kinetics of CB constituents and relate them to their functions. Coilin and SMN complex members exhibit relatively long CB residence times, whereas components of snRNPs, small nucleolar RNPs, and factors shared with the nucleolus have significantly shorter residence times. Comparison of the dissociation kinetics of these shared proteins from either the nucleolus or the CB suggests the existence of compartment-specific retention mechanisms. The dynamic properties of several CB components do not depend on their interaction with coilin because their dissociation kinetics are unaltered in residual nuclear bodies of coilin knockout cells. Photobleaching and fluorescence resonance energy transfer experiments demonstrate that coilin and SMN can interact within CBs, but their interaction is not the major determinant of their residence times. These results suggest that CBs and gems are kinetically independent structures.

Original languageEnglish (US)
Pages (from-to)831-842
Number of pages12
JournalJournal of Cell Biology
Volume164
Issue number6
DOIs
StatePublished - Mar 15 2004

Fingerprint

SMN Complex Proteins
Small Nuclear Ribonucleoproteins
Photobleaching
Fluorescence Resonance Energy Transfer
Motor Neurons
Microscopy
Proteins

Keywords

  • Cajal body
  • Coilin
  • Gems
  • iFRAP
  • SMN

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dundr, M., Hebert, M. D., Karpova, T. S., Stanek, D., Xu, H., Shpargel, K. B., ... Misteli, T. (2004). In vivo kinetics of Cajal body components. Journal of Cell Biology, 164(6), 831-842. https://doi.org/10.1083/jcb.200311121

In vivo kinetics of Cajal body components. / Dundr, Miroslav; Hebert, Michael D.; Karpova, Tatiana S.; Stanek, David; Xu, Hongzi; Shpargel, Karl B.; Meier, U. Thomas; Neugebauer, Karla M.; Matera, A. Gregory; Misteli, Tom.

In: Journal of Cell Biology, Vol. 164, No. 6, 15.03.2004, p. 831-842.

Research output: Contribution to journalArticle

Dundr, M, Hebert, MD, Karpova, TS, Stanek, D, Xu, H, Shpargel, KB, Meier, UT, Neugebauer, KM, Matera, AG & Misteli, T 2004, 'In vivo kinetics of Cajal body components', Journal of Cell Biology, vol. 164, no. 6, pp. 831-842. https://doi.org/10.1083/jcb.200311121
Dundr M, Hebert MD, Karpova TS, Stanek D, Xu H, Shpargel KB et al. In vivo kinetics of Cajal body components. Journal of Cell Biology. 2004 Mar 15;164(6):831-842. https://doi.org/10.1083/jcb.200311121
Dundr, Miroslav ; Hebert, Michael D. ; Karpova, Tatiana S. ; Stanek, David ; Xu, Hongzi ; Shpargel, Karl B. ; Meier, U. Thomas ; Neugebauer, Karla M. ; Matera, A. Gregory ; Misteli, Tom. / In vivo kinetics of Cajal body components. In: Journal of Cell Biology. 2004 ; Vol. 164, No. 6. pp. 831-842.
@article{5126aa974bb9475ba5ccc84bb56f64e7,
title = "In vivo kinetics of Cajal body components",
abstract = "Cajal bodies (CBs) are subnuclear domains implicated in small nuclear ribonucleoprotein (snRNP) biogenesis. In most cell types, CBs coincide with nuclear gems, which contain the survival of motor neurons (SMN) complex, an essential snRNP assembly factor. Here, we analyze the exchange kinetics of multiple components of CBs and gems in living cells using photobleaching microscopy. We demonstrate differences in dissociation kinetics of CB constituents and relate them to their functions. Coilin and SMN complex members exhibit relatively long CB residence times, whereas components of snRNPs, small nucleolar RNPs, and factors shared with the nucleolus have significantly shorter residence times. Comparison of the dissociation kinetics of these shared proteins from either the nucleolus or the CB suggests the existence of compartment-specific retention mechanisms. The dynamic properties of several CB components do not depend on their interaction with coilin because their dissociation kinetics are unaltered in residual nuclear bodies of coilin knockout cells. Photobleaching and fluorescence resonance energy transfer experiments demonstrate that coilin and SMN can interact within CBs, but their interaction is not the major determinant of their residence times. These results suggest that CBs and gems are kinetically independent structures.",
keywords = "Cajal body, Coilin, Gems, iFRAP, SMN",
author = "Miroslav Dundr and Hebert, {Michael D.} and Karpova, {Tatiana S.} and David Stanek and Hongzi Xu and Shpargel, {Karl B.} and Meier, {U. Thomas} and Neugebauer, {Karla M.} and Matera, {A. Gregory} and Tom Misteli",
year = "2004",
month = "3",
day = "15",
doi = "10.1083/jcb.200311121",
language = "English (US)",
volume = "164",
pages = "831--842",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "6",

}

TY - JOUR

T1 - In vivo kinetics of Cajal body components

AU - Dundr, Miroslav

AU - Hebert, Michael D.

AU - Karpova, Tatiana S.

AU - Stanek, David

AU - Xu, Hongzi

AU - Shpargel, Karl B.

AU - Meier, U. Thomas

AU - Neugebauer, Karla M.

AU - Matera, A. Gregory

AU - Misteli, Tom

PY - 2004/3/15

Y1 - 2004/3/15

N2 - Cajal bodies (CBs) are subnuclear domains implicated in small nuclear ribonucleoprotein (snRNP) biogenesis. In most cell types, CBs coincide with nuclear gems, which contain the survival of motor neurons (SMN) complex, an essential snRNP assembly factor. Here, we analyze the exchange kinetics of multiple components of CBs and gems in living cells using photobleaching microscopy. We demonstrate differences in dissociation kinetics of CB constituents and relate them to their functions. Coilin and SMN complex members exhibit relatively long CB residence times, whereas components of snRNPs, small nucleolar RNPs, and factors shared with the nucleolus have significantly shorter residence times. Comparison of the dissociation kinetics of these shared proteins from either the nucleolus or the CB suggests the existence of compartment-specific retention mechanisms. The dynamic properties of several CB components do not depend on their interaction with coilin because their dissociation kinetics are unaltered in residual nuclear bodies of coilin knockout cells. Photobleaching and fluorescence resonance energy transfer experiments demonstrate that coilin and SMN can interact within CBs, but their interaction is not the major determinant of their residence times. These results suggest that CBs and gems are kinetically independent structures.

AB - Cajal bodies (CBs) are subnuclear domains implicated in small nuclear ribonucleoprotein (snRNP) biogenesis. In most cell types, CBs coincide with nuclear gems, which contain the survival of motor neurons (SMN) complex, an essential snRNP assembly factor. Here, we analyze the exchange kinetics of multiple components of CBs and gems in living cells using photobleaching microscopy. We demonstrate differences in dissociation kinetics of CB constituents and relate them to their functions. Coilin and SMN complex members exhibit relatively long CB residence times, whereas components of snRNPs, small nucleolar RNPs, and factors shared with the nucleolus have significantly shorter residence times. Comparison of the dissociation kinetics of these shared proteins from either the nucleolus or the CB suggests the existence of compartment-specific retention mechanisms. The dynamic properties of several CB components do not depend on their interaction with coilin because their dissociation kinetics are unaltered in residual nuclear bodies of coilin knockout cells. Photobleaching and fluorescence resonance energy transfer experiments demonstrate that coilin and SMN can interact within CBs, but their interaction is not the major determinant of their residence times. These results suggest that CBs and gems are kinetically independent structures.

KW - Cajal body

KW - Coilin

KW - Gems

KW - iFRAP

KW - SMN

UR - http://www.scopus.com/inward/record.url?scp=12144290254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12144290254&partnerID=8YFLogxK

U2 - 10.1083/jcb.200311121

DO - 10.1083/jcb.200311121

M3 - Article

VL - 164

SP - 831

EP - 842

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 6

ER -