In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis

Agriculture & Biology

• Mycobacterium bovis BCG 100%
• auxotrophs 84%
• mutagenesis 66%
• transposons 63%
• leucine 62%
• kanamycin 21%
• Mycobacterium tuberculosis complex 14%
• insertional mutagenesis 13%
• clones 13%
• Mycobacterium smegmatis 13%
• transposition (genetics) 11%
• Cyanobacteria 8%
• resistance genes 8%
• binding proteins 8%
• sulfates 8%
• spleen 8%
• open reading frames 7%
• lungs 7%
• plasmids 7%
• sequence analysis 7%
• Escherichia coli 6%
• mice 5%
• genome 5%
• genes 3%

Medicine & Life Sciences

• Mycobacterium bovis 74%
• Methionine 72%
• Leucine 71%
• Mutagenesis 68%
• Growth 34%
• Libraries 20%
• Clone Cells 18%
• Kanamycin Resistance 17%
• Mycobacterium smegmatis 16%
• Kanamycin 15%
• Cyanobacteria 15%
• Insertional Mutagenesis 13%
• Sulfates 12%
• Open Reading Frames 11%
• Inbred C57BL Mouse 10%
• Sequence Analysis 10%
• Carrier Proteins 9%
• Tuberculosis 9%
• Plasmids 9%
• Genes 9%
• Spleen 8%
• Escherichia coli 8%
• Genome 7%
• Lung 6%