In vivo and in vitro regulation of hepatic glucagon receptor mRNA concentration by glucose metabolism

Rémy Burcelin, Caroline Mrejen, Jean Francois Decaux, Sylvie Hauguel De Mouzon, Jean Girard, Maureen J. Charron

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have recently cloned the murine glucagon receptor (GR) gene and shown that it is expressed mainly in liver. In this organ, the glucagon-GR system is involved in the control of glucose metabolism as it initiates a cascade of events leading to release of glucose into the blood stream, which is a main feature in several physiological and pathological conditions. To better define the metabolic regulators of GR expression in liver we analyzed GR mRNA concentration in physiological conditions associating various glucose metabolic pathways in vivo and in vitro in the rat and in the mouse. First, we report that the concentration of the GR mRNA progressively increased from the first day of life to the adult stage. This effect was abolished when newborn rodents were fasted. Second, under conditions where intrahepatic glucose metabolism was active such as during fasting, diabetes, and hyperglycemic clamp, the concentration of GR mRNA increased independent of the origin of the pathway that generated the glucose flux. These effects were blunted when hyperglycemia was corrected by phlorizin treatment of diabetic rats or not sustained during euglycemic clamp. In accordance with these observations, we demonstrated that the glycolytic substrates glucose, mannose, and fructose, as well as the gluconeognic substrates glycerol and dihydroxyacetone, increased the concentration of GR mRNA in primary cultures of hepatocytes from fed rats. Glucagon blunted the effect of glucose without being dominant. The stimulatory effect of those substrates was not mimicked by the nonmetabolizable carbohydrate L-glucose or the glucokinase inhibitor glucosamine or when hepatocytes were isolated from starved rats. In addition, inhibitors of gluconeogenesis and lipolysis could decrease the concentration of GR mRNA from hepatocytes of starved rats. Combined, these data strongly suggest that glucose flux in the glycolytic and gluconeogenic pathways at the level of triose intermediates could control expression of GR mRNA and participate in controlling its own metabolism.

Original languageEnglish (US)
Pages (from-to)8088-8093
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number14
DOIs
StatePublished - Apr 3 1998

Fingerprint

Glucagon Receptors
Metabolism
Glucose
Messenger RNA
Liver
Rats
Hepatocytes
Clamping devices
Glucagon
Substrates
Trioses
Dihydroxyacetone
Phlorhizin
Fluxes
Glucokinase
In Vitro Techniques
Glucose Clamp Technique
Gluconeogenesis
Lipolysis
Glucosamine

ASJC Scopus subject areas

  • Biochemistry

Cite this

In vivo and in vitro regulation of hepatic glucagon receptor mRNA concentration by glucose metabolism. / Burcelin, Rémy; Mrejen, Caroline; Decaux, Jean Francois; De Mouzon, Sylvie Hauguel; Girard, Jean; Charron, Maureen J.

In: Journal of Biological Chemistry, Vol. 273, No. 14, 03.04.1998, p. 8088-8093.

Research output: Contribution to journalArticle

Burcelin, Rémy ; Mrejen, Caroline ; Decaux, Jean Francois ; De Mouzon, Sylvie Hauguel ; Girard, Jean ; Charron, Maureen J. / In vivo and in vitro regulation of hepatic glucagon receptor mRNA concentration by glucose metabolism. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 14. pp. 8088-8093.
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AU - Mrejen, Caroline

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AU - Charron, Maureen J.

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