In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Ousmane Sarr, Alissa Myrick, Johanna P. Daily, Bernard M. Diop, Therese Dieng, Omar Ndir, Pape Salif Sow, Souleymane Mboup, Dyann F. Wirth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

Original languageEnglish (US)
Pages (from-to)136-140
Number of pages5
JournalParasitology Research
Volume97
Issue number2
DOIs
StatePublished - Aug 2005
Externally publishedYes

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chloroquine
Senegal
Chloroquine
Plasmodium falciparum
Alleles
alleles
genetic polymorphism
loci
Parasitemia
parasitemia
In Vitro Techniques
genetic markers
DNA

ASJC Scopus subject areas

  • Parasitology

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In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal. / Sarr, Ousmane; Myrick, Alissa; Daily, Johanna P.; Diop, Bernard M.; Dieng, Therese; Ndir, Omar; Sow, Pape Salif; Mboup, Souleymane; Wirth, Dyann F.

In: Parasitology Research, Vol. 97, No. 2, 08.2005, p. 136-140.

Research output: Contribution to journalArticle

Sarr, O, Myrick, A, Daily, JP, Diop, BM, Dieng, T, Ndir, O, Sow, PS, Mboup, S & Wirth, DF 2005, 'In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal', Parasitology Research, vol. 97, no. 2, pp. 136-140. https://doi.org/10.1007/s00436-005-1406-7
Sarr, Ousmane ; Myrick, Alissa ; Daily, Johanna P. ; Diop, Bernard M. ; Dieng, Therese ; Ndir, Omar ; Sow, Pape Salif ; Mboup, Souleymane ; Wirth, Dyann F. / In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal. In: Parasitology Research. 2005 ; Vol. 97, No. 2. pp. 136-140.
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abstract = "To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17{\%} of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64{\%} of all isolates had the T76 resistant allele at the pfcrt locus, while 30{\%} carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89{\%} of in vitro resistant isolates, but also in 35{\%} of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.",
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AU - Dieng, Therese

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AU - Sow, Pape Salif

AU - Mboup, Souleymane

AU - Wirth, Dyann F.

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