In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Ousmane Sarr; Alissa Myrick; Johanna Daily; Bernard M. Diop; Therese Dieng; Omar Ndir; Pape Salif Sow; Souleymane Mboup; Dyann F. Wirth

doi:10.1007/s00436-005-1406-7

In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Ousmane Sarr, Alissa Myrick, Johanna Daily, Bernard M. Diop, Therese Dieng, Omar Ndir, Pape Salif Sow, Souleymane Mboup, Dyann F. Wirth

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

Original language	English (US)
Pages (from-to)	136-140
Number of pages	5
Journal	Parasitology research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1007/s00436-005-1406-7
State	Published - Aug 2005
Externally published	Yes

ASJC Scopus subject areas

Parasitology
General Veterinary
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-005-1406-7

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title = "In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal",

abstract = "To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.",

author = "Ousmane Sarr and Alissa Myrick and Johanna Daily and Diop, {Bernard M.} and Therese Dieng and Omar Ndir and Sow, {Pape Salif} and Souleymane Mboup and Wirth, {Dyann F.}",

note = "Funding Information: Acknowledgements We are grateful to the cooperation between HSPH and Cheikh Anta Diop University. We thank Abdoulaye Diallo, the patients from whom blood samples were drawn and the health workers at Pikine for their assistance. Dr. Fariba Houman, Dr. Connie Chow, and Kevin Rader are thanked for their help in reviewing the manuscript and in help with statistical data analysis. The work was financial supported by Fogarty International Research Training Grant D43 TW01503, The Ellison Medical Foundation, The Exxon Mobil Corporation, The National Institutes of Health Grant 1RO1 GM61351, and the Harvard Malaria Initiative supported this study that was performed in compliance with research regulatory laws in USA and Senegal.",

year = "2005",

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doi = "10.1007/s00436-005-1406-7",

language = "English (US)",

volume = "97",

pages = "136--140",

journal = "Parasitology research",

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T1 - In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

AU - Sarr, Ousmane

AU - Myrick, Alissa

AU - Daily, Johanna

AU - Diop, Bernard M.

AU - Dieng, Therese

AU - Ndir, Omar

AU - Sow, Pape Salif

AU - Mboup, Souleymane

AU - Wirth, Dyann F.

N1 - Funding Information: Acknowledgements We are grateful to the cooperation between HSPH and Cheikh Anta Diop University. We thank Abdoulaye Diallo, the patients from whom blood samples were drawn and the health workers at Pikine for their assistance. Dr. Fariba Houman, Dr. Connie Chow, and Kevin Rader are thanked for their help in reviewing the manuscript and in help with statistical data analysis. The work was financial supported by Fogarty International Research Training Grant D43 TW01503, The Ellison Medical Foundation, The Exxon Mobil Corporation, The National Institutes of Health Grant 1RO1 GM61351, and the Harvard Malaria Initiative supported this study that was performed in compliance with research regulatory laws in USA and Senegal.

PY - 2005/8

Y1 - 2005/8

N2 - To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

AB - To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

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In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

Abstract

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