In vitro transcriptional activation by a metabolic intermediate: Activation by Leu3 depends on α-isopropylmalate

Ji Ying Sze, Michael Woontner, Judith A. Jaehning, Gunter B. Kohlhaw

Research output: Contribution to journalArticle

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Abstract

In the absence of the leucine biosynthetic precursor α- isopropylmalate (α-IPM), the yeast LEU3 protein (Leu3p) binds DNA and acts as a transcriptional repressor in an in vitro extract. Addition of α-IPM resulted in a dramatic increase in Leu3p-dependent transcription. The presence of α-IPM was also required for Leu3p to compete effectively with another transcriptional activator, GAL4/VP16, for limiting transcription factors. Therefore, the addition of α-IPM appears to convert a transcriptional repressor into an activator. This represents an example in eukaryotes of direct transcriptional regulation by a small effector molecule.

Original languageEnglish (US)
Pages (from-to)1143-1145
Number of pages3
JournalScience
Volume258
Issue number5085
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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