In vitro transcriptional activation by a metabolic intermediate

Activation by Leu3 depends on α-isopropylmalate

Ji Ying Sze, Michael Woontner, Judith A. Jaehning, Gunter B. Kohlhaw

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

In the absence of the leucine biosynthetic precursor α-isopropylmalate (α-IPM), the yeast LEU3 protein (Leu3p) binds DNA and acts as a transcriptional repressor in an in vitro extract. Addition of α-IPM resulted in a dramatic increase in Leu3p-dependent transcription. The presence of α-IPM was also required for Leu3p to compete effectively with another transcriptional activator, GAL4/VP16, for limiting transcription factors. Therefore, the addition of α-IPM appears to convert a transcriptional repressor into an activator. This represents an example in eukaryotes of direct transcriptional regulation by a small effector molecule.

Original languageEnglish (US)
Pages (from-to)1143-1145
Number of pages3
JournalScience
Volume258
Issue number5085
StatePublished - Nov 13 1992
Externally publishedYes

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Transcriptional Activation
Fungal Proteins
Eukaryota
Leucine
Proteins
Transcription Factors
DNA
Metabolic Activation
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

In vitro transcriptional activation by a metabolic intermediate : Activation by Leu3 depends on α-isopropylmalate. / Sze, Ji Ying; Woontner, Michael; Jaehning, Judith A.; Kohlhaw, Gunter B.

In: Science, Vol. 258, No. 5085, 13.11.1992, p. 1143-1145.

Research output: Contribution to journalArticle

Sze, JY, Woontner, M, Jaehning, JA & Kohlhaw, GB 1992, 'In vitro transcriptional activation by a metabolic intermediate: Activation by Leu3 depends on α-isopropylmalate', Science, vol. 258, no. 5085, pp. 1143-1145.
Sze, Ji Ying ; Woontner, Michael ; Jaehning, Judith A. ; Kohlhaw, Gunter B. / In vitro transcriptional activation by a metabolic intermediate : Activation by Leu3 depends on α-isopropylmalate. In: Science. 1992 ; Vol. 258, No. 5085. pp. 1143-1145.
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