In vitro glycolysis of whole blood can detect primed neutrophils in septic icu patients

David M. Nierman, Thomas H. Kalb, Clyde B. Schechter

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The purpose of this study was to develop a bedside assay based on the in vitro glycolysis of a whole blood sample that could detect primed neutrophils (PMNs). A mathematical index of the PMN response to exogenous stimulation with phorbol myristate 13-acetate (PMA), called the Delta value, was derived by comparing the increase in glycolysis for paired blood samples with and without PMA to that expected from normal subjects. Delta values for systemic inflammatory response syndrome/sepsis patients (9.09 ± 7.61) (N = 36) were significantly higher than normal controls (2.02 ± 1.76) (N = 51), nonsepsis ICU patients (3.81 ± 2.80) (N = 14) and patients in septic shock (2.33 ± 3.04) (N = 10) (p <.05). Delta values were consistently reflected in parallel measurements of increased reactive oxygen species production by neutrophils detected cytofluorometrically. PMN priming can be simply and rapidly detected by an assay based on the numbers of PMNs and erythrocytes and the measured rates of in vitro glycolysis of paired whole blood samples with and without PMA.

Original languageEnglish (US)
Pages (from-to)88-95
Number of pages8
JournalShock
Volume3
Issue number2
StatePublished - 1995
Externally publishedYes

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Glycolysis
Neutrophils
Tetradecanoylphorbol Acetate
Systemic Inflammatory Response Syndrome
Septic Shock
Reactive Oxygen Species
Sepsis
Erythrocytes
In Vitro Techniques

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Physiology

Cite this

In vitro glycolysis of whole blood can detect primed neutrophils in septic icu patients. / Nierman, David M.; Kalb, Thomas H.; Schechter, Clyde B.

In: Shock, Vol. 3, No. 2, 1995, p. 88-95.

Research output: Contribution to journalArticle

Nierman, David M. ; Kalb, Thomas H. ; Schechter, Clyde B. / In vitro glycolysis of whole blood can detect primed neutrophils in septic icu patients. In: Shock. 1995 ; Vol. 3, No. 2. pp. 88-95.
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