In vitro generation of natural killer cells from SJL/J bone marrow precursors

L. D'Adamio, L. Cannarile, G. Migliorati, C. Riccardi

Research output: Contribution to journalArticle

Abstract

The development of natural killer (NK) cells from undifferentiated bone marrow (BM) precursors of low-NK-reactive SJL/J mice was studied. Results indicate that BM cells of untreated mice are not able to generate NK effector cells in cultures supplemented with recombinant interleukin-2 (IL-2). On the other hand in the presence of IL-2, NK cells are generated in cultures of BM from mice pretreated with 5-fluorouracil (5-FU, 150 mg/kg iv 4 days before harvesting), a treatment which has been shown to eliminate more differentiated but spare less differentiated BM precursors. The 5-FU resistant BM progenitor is asialoGM1-, Thy.1+, Lyt.1- and Lyt.2-. The cells generated by culturing with IL-2 are asialoGM1+, Thy.1+, Lyt.5+, Lyt.1-, Lyt.2- and lyse only NK-susceptible targets. Generation of NK cells is blocked by addition of anti-IL-2 receptor (IL-2/r) antibodies. These studies demonstrate that it is possible to generate NK effectors from SJL/J BM cells by in vitro culturing with IL-2.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalJournal of Biological Regulators and Homeostatic Agents
Volume2
Issue number2
StatePublished - 1988
Externally publishedYes

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natural killer cells
interleukin-2
Natural Killer Cells
bone marrow
Interleukin-2
Bone Marrow
Fluorouracil
Bone Marrow Cells
bone marrow cells
mice
Interleukin-2 Receptors
fluorouracil
Cell Culture Techniques
cell culture
In Vitro Techniques
Antibodies
receptors
antibodies
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)

Cite this

In vitro generation of natural killer cells from SJL/J bone marrow precursors. / D'Adamio, L.; Cannarile, L.; Migliorati, G.; Riccardi, C.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 2, No. 2, 1988, p. 71-76.

Research output: Contribution to journalArticle

D'Adamio, L, Cannarile, L, Migliorati, G & Riccardi, C 1988, 'In vitro generation of natural killer cells from SJL/J bone marrow precursors', Journal of Biological Regulators and Homeostatic Agents, vol. 2, no. 2, pp. 71-76.
D'Adamio, L. ; Cannarile, L. ; Migliorati, G. ; Riccardi, C. / In vitro generation of natural killer cells from SJL/J bone marrow precursors. In: Journal of Biological Regulators and Homeostatic Agents. 1988 ; Vol. 2, No. 2. pp. 71-76.
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