IN-VITRO-DIFFERENZIERUNG LEUKAMISCHER PROMYELOZYTEN (HL-60): VERHALTEN DER β-ADRENERGEN REZEPTOREN UND DER ADENYLATZYKLASEAKTIVITAT

Translated title of the contribution: In vitro differentiation of leukaemic promyelocytes (HL-60): Effect on β-adrenergic receptors and adenylate cyclase activity

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

In the present study, the expression of β-adrenergic receptors, the activity of the enzyme adenylate cyclase (AC), and the intracellular concentration of cyclic adenosine 3',5'-monophosphate (cAMP) were investigated in HL-60 cells before and after their differentiation to monocytic or more mature granulocytic cells. Treatment of the HL-60 promyelocytes with 12-O-tetradecanoylphorbol 13-acetate or human interleukin 2 resulted in the appearance of cells with monocytic characteristics (morphology, non-specific esterase, adherence, reaction with a monocyte-specific monoclonal antibody). Induction with retinoic acid resulted in differentiation to cells with typical features of mature granulocytes (morphology, peroxidase, nitroblue tetrazolium reduction). During maturation to monocytes, the density of β-adrenergic receptors decreased, whereas it remained constant during maturation to granulocytes. In comparison with normal circulating monocytes or polymorphonuclear granulocytes, expression of β-adrenergic receptors on the surface of the differentiated HL-60 cells was low. Activation of AC by the hormones isoproterenol, prostaglandin E1, and histamine generally decreased with HL-60 maturation and enzyme activities were markedly below those measured in normal peripheral leukocytes. In the induced monocytic HL-60 cells, the weak effectiveness of isoproterenol was due to the loss of β-adrenergic receptors. In the induced granulocytic HL-60 cells, the reduced hormonal AC activation could be explained by the impaired coupling between hormone receptors and catalytic enzyme unit. The concentration of intracellular cAMP after differentiation of HL-60 cells reflected the increase in basal AC activity and the decrease in hormone stimulation of the enzyme. Our data indicate that HL-60 cells induced to differentiate possess some monocyte- and granulocyte-like properties, but do not meet several important functional criteria of their new cell identities.

Original languageGerman
Pages (from-to)336-344
Number of pages9
JournalWiener Klinische Wochenschrift
Volume95
Issue number10
StatePublished - Jan 1 1983
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Medicine(all)

Cite this