TY - JOUR
T1 - In vitro derivation of macrophage from guinea pig bone marrow with human M-CSF
AU - Yu, Karl O.A.
AU - Porcelli, Steven A.
AU - Shuman, Howard A.
N1 - Funding Information:
We thank L. Zitzow (Department of Surgery, University of Chicago) and L. Degenstein (Transgenic Core, University of Chicago) for generous provision of guinea pigs and C57Bl/6 mice. S. cerevisiae was a kind gift of T. Bishop and J. Grub. S. Matushek (University of Chicago Medical Center Clinical Microbiology Laboratory) kindly provided India ink. We appreciate extended helpful discussions with several members of the laboratory, and thank A. Punnoose and A. Jongco for critique of the manuscript. K.Y. is supported by the Infectious Diseases Fellowship Program of the Department of Pediatrics at Comer Children's Hospital. This work was supported by the Department of Microbiology at the University of Chicago .
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/3/29
Y1 - 2013/3/29
N2 - The guinea pig has a storied history as a model in the study of infectious disease and immunology. Because of reproducibility of data and availability of various reagents, inbred mice have since supplanted the guinea pig as the animal model-of-choice in these fields. However, several clinically-significant microorganisms do not cause the same pathology in mice, or mice may not be susceptible to these infections. These demonstrate the utility of other animal models - either as the primary method to study a particular infection, or to confirm or refute findings in the mouse before translating basic science into clinical practice.The mononuclear phagocyte, or macrophage (Mφ), plays a key role in antigen presentation and the pathogenesis of intracellular bacteria, such as Mycobacterium tuberculosis and Legionella pneumophila. Because of variable yield and difficult extraction from tissue, the preferred method of producing Mφ for in vitro studies is to expand murine bone marrow (BM) precursors with mouse macrophage colony-stimulating factor (M-CSF). This has not been shown in the guinea pig. Here, we report the empiric observation that human M-CSF - but not mouse M-CSF, nor human granulocyte/macrophage colony-stimulating factor - can be used to induce BM precursor differentiation into bonafide Mφ. The differentiated cells appeared as enlarged adherent cells, capable of both pinocytosis and large particle phagocytosis. Furthermore, we showed that these guinea pig BM-derived Mφ, similar to human monocyte/Mφ lines but unlike most murine BM Mφ, support growth of wild type L. pneumophila.This method may prove useful for in vitro studies of Mφ in the guinea pig, as well as in the translation of results found using mouse BM-derived Mφ towards studies in human immunology and infectious disease.
AB - The guinea pig has a storied history as a model in the study of infectious disease and immunology. Because of reproducibility of data and availability of various reagents, inbred mice have since supplanted the guinea pig as the animal model-of-choice in these fields. However, several clinically-significant microorganisms do not cause the same pathology in mice, or mice may not be susceptible to these infections. These demonstrate the utility of other animal models - either as the primary method to study a particular infection, or to confirm or refute findings in the mouse before translating basic science into clinical practice.The mononuclear phagocyte, or macrophage (Mφ), plays a key role in antigen presentation and the pathogenesis of intracellular bacteria, such as Mycobacterium tuberculosis and Legionella pneumophila. Because of variable yield and difficult extraction from tissue, the preferred method of producing Mφ for in vitro studies is to expand murine bone marrow (BM) precursors with mouse macrophage colony-stimulating factor (M-CSF). This has not been shown in the guinea pig. Here, we report the empiric observation that human M-CSF - but not mouse M-CSF, nor human granulocyte/macrophage colony-stimulating factor - can be used to induce BM precursor differentiation into bonafide Mφ. The differentiated cells appeared as enlarged adherent cells, capable of both pinocytosis and large particle phagocytosis. Furthermore, we showed that these guinea pig BM-derived Mφ, similar to human monocyte/Mφ lines but unlike most murine BM Mφ, support growth of wild type L. pneumophila.This method may prove useful for in vitro studies of Mφ in the guinea pig, as well as in the translation of results found using mouse BM-derived Mφ towards studies in human immunology and infectious disease.
KW - Bone marrow
KW - CSF-1
KW - Guinea pig
KW - M-CSF
KW - Macrophage
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U2 - 10.1016/j.jim.2013.01.005
DO - 10.1016/j.jim.2013.01.005
M3 - Article
C2 - 23333710
AN - SCOPUS:84873706546
SN - 0022-1759
VL - 389
SP - 88
EP - 94
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -