In vitro assessment of antimicrobial agents against Toxoplasma gondii

C. Harris, M. P. Salgo, H. B. Tanowitz, M. Wittner

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We have modified a method of quantitating growth of Toxoplasma organisms by measuring incorporation of [3H]uracil into Toxoplasma-infected, differentiated L6E9 rat myocytes and have found that low-dose pyrimethamine (0.1 μg/ml) and sulfadiazine (25 μg/ml) are synergistic. Pyrimethamine at higher concentrations (0.5 and 1.0 μg/ml) inhibits uptake to the same degree as the low-dose pyrimethamine-sulfadiazine combination. Spiramycin was effective only at high concentrations (200 μg/ml) and with prolonged incubation of > 72 h. Clindamycin and several of its analogues, methotrexate and difluoromethylornithine, were all ineffective and showed no additive effect with either pyrimethamine or sulfadiazine. Spirogermanium, an experimental antineoplastic and antiprotozoan agent, was effective only at concentrations close to those toxic to the system. 5-Fluorouracil was effective even at 0.1 μg/ml. At 0.01 μg/ml it was synergistic with pyrimethamine (0.1 μg/ml), and the combination was as effective as high-dose pyrimethamine (1.0 μg/ml).

Original languageEnglish (US)
Pages (from-to)14-22
Number of pages9
JournalJournal of Infectious Diseases
Volume157
Issue number1
StatePublished - 1988

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Pyrimethamine
Toxoplasma
Anti-Infective Agents
Sulfadiazine
spirogermanium
Spiramycin
Eflornithine
Uracil
Clindamycin
Poisons
Methotrexate
Fluorouracil
Antineoplastic Agents
Muscle Cells
In Vitro Techniques
Growth

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Harris, C., Salgo, M. P., Tanowitz, H. B., & Wittner, M. (1988). In vitro assessment of antimicrobial agents against Toxoplasma gondii. Journal of Infectious Diseases, 157(1), 14-22.

In vitro assessment of antimicrobial agents against Toxoplasma gondii. / Harris, C.; Salgo, M. P.; Tanowitz, H. B.; Wittner, M.

In: Journal of Infectious Diseases, Vol. 157, No. 1, 1988, p. 14-22.

Research output: Contribution to journalArticle

Harris, C, Salgo, MP, Tanowitz, HB & Wittner, M 1988, 'In vitro assessment of antimicrobial agents against Toxoplasma gondii', Journal of Infectious Diseases, vol. 157, no. 1, pp. 14-22.
Harris C, Salgo MP, Tanowitz HB, Wittner M. In vitro assessment of antimicrobial agents against Toxoplasma gondii. Journal of Infectious Diseases. 1988;157(1):14-22.
Harris, C. ; Salgo, M. P. ; Tanowitz, H. B. ; Wittner, M. / In vitro assessment of antimicrobial agents against Toxoplasma gondii. In: Journal of Infectious Diseases. 1988 ; Vol. 157, No. 1. pp. 14-22.
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