In vitro and in vivo osteogenic differentiation of human adipose-derived stromal cells

Clement D. Marshall, Elizabeth A. Brett, Alessandra L. Moore, Derrick C. Wan, Michael T. Longaker

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Adipose-derived stromal cells (ASCs) are a promising population of cells that may be useful for the regeneration of human tissue defects. ASCs are capable of forming bone tissue in vitro and in vivo. Further work is required to determine the optimal conditions that will allow human ASCs to regenerate tissue in clinically significant tissue defects. Here we present three experimental protocols that are indispensable for the study of ASC osteogenic activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages9-18
Number of pages10
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1891
ISSN (Print)1064-3745

Keywords

  • Adipose-derived stromal cell
  • Bone defect
  • Calvarial defect
  • Osteogenesis
  • Osteogenic differentiation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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