TY - JOUR
T1 - In Vitro and In Vivo Genetic Disease Modeling via NHEJ-Precise Deletions Using CRISPR-Cas9
AU - López-Manzaneda, Sergio
AU - Ojeda-Pérez, Isabel
AU - Zabaleta, Nerea
AU - García-Torralba, Aída
AU - Alberquilla, Omaira
AU - Torres, Raúl
AU - Sánchez-Domínguez, Rebeca
AU - Torella, Laura
AU - Olivier, Emmanuel
AU - Mountford, Joanne
AU - Ramírez, Juan C.
AU - Bueren, Juan A.
AU - González-Aseguinolaza, Gloria
AU - Segovia, Jose Carlos
N1 - Publisher Copyright:
© 2020 The Authors
PY - 2020/12/11
Y1 - 2020/12/11
N2 - A CRISPR-Cas9 system generates precise deletions (up to 90% efficiency) when two guides are used simultaneously by means of non-homologous end joining. The PAM-in/PAM-in Cas9 orientation is the best combination to generate precise deletions. This gene-editing procedure facilitates the generation of homogeneously genetically defined cell and animal models.
AB - A CRISPR-Cas9 system generates precise deletions (up to 90% efficiency) when two guides are used simultaneously by means of non-homologous end joining. The PAM-in/PAM-in Cas9 orientation is the best combination to generate precise deletions. This gene-editing procedure facilitates the generation of homogeneously genetically defined cell and animal models.
UR - http://www.scopus.com/inward/record.url?scp=85097045636&partnerID=8YFLogxK
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U2 - 10.1016/j.omtm.2020.10.007
DO - 10.1016/j.omtm.2020.10.007
M3 - Article
AN - SCOPUS:85097045636
SN - 2329-0501
VL - 19
SP - 426
EP - 437
JO - Molecular Therapy Methods and Clinical Development
JF - Molecular Therapy Methods and Clinical Development
ER -