A CRISPR-Cas9 system generates precise deletions (up to 90% efficiency) when two guides are used simultaneously by means of non-homologous end joining. The PAM-in/PAM-in Cas9 orientation is the best combination to generate precise deletions. This gene-editing procedure facilitates the generation of homogeneously genetically defined cell and animal models.
|Original language||English (US)|
|Number of pages||12|
|Journal||Molecular Therapy - Methods and Clinical Development|
|State||Published - Dec 11 2020|
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology