In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion

B. J. Kobrin, C. Schiff, D. Zivion, Matthew D. Scharff, G. Spira

Research output: Contribution to journalArticle

Abstract

We have shown that one of the alleles in a hybridoma was nonproductive because the sequence in the N region of the heavy chain caused it to be out of frame and to terminate prematurely. This allele became productive, and the γ1 heavy chain that it encoded was secreted when an adenosine was inserted to produce an open reading frame. This event occurred at a very low frequency following mutagenesis of the cultured cells. This result suggests that similar sorts of events must occur in vivo when both productive and nonproductive alleles undergo frequent mutations and that new genes may be expressed in hybridomas as they are being subcloned.

Original languageEnglish (US)
Pages (from-to)257-261
Number of pages5
JournalHybridoma
Volume13
Issue number4
StatePublished - 1994

Fingerprint

Base Pairing
Immunoglobulins
Alleles
Hybridomas
Mutagenesis
Adenosine
Open Reading Frames
Cultured Cells
Mutation
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Kobrin, B. J., Schiff, C., Zivion, D., Scharff, M. D., & Spira, G. (1994). In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion. Hybridoma, 13(4), 257-261.

In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion. / Kobrin, B. J.; Schiff, C.; Zivion, D.; Scharff, Matthew D.; Spira, G.

In: Hybridoma, Vol. 13, No. 4, 1994, p. 257-261.

Research output: Contribution to journalArticle

Kobrin, BJ, Schiff, C, Zivion, D, Scharff, MD & Spira, G 1994, 'In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion', Hybridoma, vol. 13, no. 4, pp. 257-261.
Kobrin, B. J. ; Schiff, C. ; Zivion, D. ; Scharff, Matthew D. ; Spira, G. / In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion. In: Hybridoma. 1994 ; Vol. 13, No. 4. pp. 257-261.
@article{7d1e4b3ec38d4c2c8529ef2b5160a867,
title = "In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion",
abstract = "We have shown that one of the alleles in a hybridoma was nonproductive because the sequence in the N region of the heavy chain caused it to be out of frame and to terminate prematurely. This allele became productive, and the γ1 heavy chain that it encoded was secreted when an adenosine was inserted to produce an open reading frame. This event occurred at a very low frequency following mutagenesis of the cultured cells. This result suggests that similar sorts of events must occur in vivo when both productive and nonproductive alleles undergo frequent mutations and that new genes may be expressed in hybridomas as they are being subcloned.",
author = "Kobrin, {B. J.} and C. Schiff and D. Zivion and Scharff, {Matthew D.} and G. Spira",
year = "1994",
language = "English (US)",
volume = "13",
pages = "257--261",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - In vitro activation of a nonproductive immunoglobulin allele by a single base pair insertion

AU - Kobrin, B. J.

AU - Schiff, C.

AU - Zivion, D.

AU - Scharff, Matthew D.

AU - Spira, G.

PY - 1994

Y1 - 1994

N2 - We have shown that one of the alleles in a hybridoma was nonproductive because the sequence in the N region of the heavy chain caused it to be out of frame and to terminate prematurely. This allele became productive, and the γ1 heavy chain that it encoded was secreted when an adenosine was inserted to produce an open reading frame. This event occurred at a very low frequency following mutagenesis of the cultured cells. This result suggests that similar sorts of events must occur in vivo when both productive and nonproductive alleles undergo frequent mutations and that new genes may be expressed in hybridomas as they are being subcloned.

AB - We have shown that one of the alleles in a hybridoma was nonproductive because the sequence in the N region of the heavy chain caused it to be out of frame and to terminate prematurely. This allele became productive, and the γ1 heavy chain that it encoded was secreted when an adenosine was inserted to produce an open reading frame. This event occurred at a very low frequency following mutagenesis of the cultured cells. This result suggests that similar sorts of events must occur in vivo when both productive and nonproductive alleles undergo frequent mutations and that new genes may be expressed in hybridomas as they are being subcloned.

UR - http://www.scopus.com/inward/record.url?scp=0028568417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028568417&partnerID=8YFLogxK

M3 - Article

C2 - 7806247

AN - SCOPUS:0028568417

VL - 13

SP - 257

EP - 261

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 4

ER -