Improved identification of Staphylococcus aureus using a new agglutination test. Results of an international study

J. Croize, P. Gialanella, D. Monnet, J. Okada, A. Orsi, A. Voss, S. Merlin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A new reagent for the identification of Staphylococcus aureus, SLIDEX STAPH-KIT, operates on the principle of a latex and red blood cell combination agglutination: red blood cells are coated with fibrinogen for the detection of clumping factor, and latex particles are sensitized with anti-S. aureus serotype 18 monoclonal antibody for the detection of protein A and antigen 18. French strains belonging to serotype 18 are methicillin-resistant. The performance of this reagent was compared with STAPHYSLIDE and STAPHAUREX in Europe (France, Germany, Italy), in the United States and in Japan using 548 methicillin-resistant S. aureus strains, 392 methicillin-sensitive S. aureus strains, and 441 non-aureus staphylococci. The specificity of the three reagents was equivalent (98.8% for SLIDEX STAPH-KIT, 99.1% for STAPHYSLIDE, 98.1% for STAPHAUREX). SLIDEX STAPH-KIT (97.3%) was more sensitive than STAPHYSLIDE (93.5%) and STAPHAUREX (89.7%) for all S. aureus strains due to a higher rate of identified methicillin-resistant S. aureus strains.

Original languageEnglish (US)
Pages (from-to)487-491
Number of pages5
JournalAPMIS
Volume101
Issue number6
StatePublished - 1993
Externally publishedYes

Fingerprint

Agglutination Tests
Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus
Erythrocytes
Methicillin Resistance
Methicillin
Agglutination
Latex
Staphylococcal Protein A
Microspheres
Staphylococcus
Fibrinogen
Italy
France
Germany
Japan
Monoclonal Antibodies
Antigens
Serogroup

Keywords

  • Agglutination test
  • Methicillin-resistant S. aureus
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Pathology and Forensic Medicine

Cite this

Croize, J., Gialanella, P., Monnet, D., Okada, J., Orsi, A., Voss, A., & Merlin, S. (1993). Improved identification of Staphylococcus aureus using a new agglutination test. Results of an international study. APMIS, 101(6), 487-491.

Improved identification of Staphylococcus aureus using a new agglutination test. Results of an international study. / Croize, J.; Gialanella, P.; Monnet, D.; Okada, J.; Orsi, A.; Voss, A.; Merlin, S.

In: APMIS, Vol. 101, No. 6, 1993, p. 487-491.

Research output: Contribution to journalArticle

Croize, J, Gialanella, P, Monnet, D, Okada, J, Orsi, A, Voss, A & Merlin, S 1993, 'Improved identification of Staphylococcus aureus using a new agglutination test. Results of an international study', APMIS, vol. 101, no. 6, pp. 487-491.
Croize, J. ; Gialanella, P. ; Monnet, D. ; Okada, J. ; Orsi, A. ; Voss, A. ; Merlin, S. / Improved identification of Staphylococcus aureus using a new agglutination test. Results of an international study. In: APMIS. 1993 ; Vol. 101, No. 6. pp. 487-491.
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