Improved heart preservation with University of Wisconsin solution

experimental and preliminary human experience.

V. Jeevanandam, J. S. Auteri, J. A. Sanchez, M. L. Barr, G. Y. Ott, Daphne T. Hsu, C. Marboe, C. R. Smith, E. A. Rose

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We tested the ability of University of Wisconsin solution (UWS) to extend hypothermic nonperfused heart preservation in baboons and then proceeded to human transplantation. Orthotopic transplantation was performed in five baboons (UWS cardioplegia and storage [4 degrees C]; preservation time 10.3 +/- 0.6 hours). Four survivors were immunosuppressed for 45 days and killed. One animal died from disruption of the aortic anastomosis due to technical error. Preservation did not alter histology under light and electron microscopy or heart weight (at harvest, 51.4 +/- 11.6 g; before implant, 52.5 +/- 11.1 g). Animals were weaned from bypass (mean, 23 +/- 12 minutes) and returned to their cages without intravenous support within 3.6 +/- 0.6 hours. Weekly biopsy, electrocardiogram, enzyme analysis, echocardiogram, and right heart catheterization demonstrated excellent cardiac function. Following success in baboons, UWS was applied to human transplantation (n = 2, UWS cardioplegia and storage [4 degrees C]; preservation time 4.2 and 2.1 hours). The hearts returned to sinus rhythm within 4 minutes of reperfusion without defibrillation, and enzymatic and hemodynamic data reveal excellent heart preservation. Preliminary data suggest the ability of UWS to prolong heart preservation in baboons and be used safely in humans. Further studies are required to compare UWS with crystalloid cardioplegia and saline storage and to prolong donor heart preservation in humans.

Original languageEnglish (US)
JournalCirculation
Volume84
Issue number5 Suppl
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Papio
Induced Heart Arrest
Transplantation
Cardiac Catheterization
Reperfusion
Histology
Electron Microscopy
Electrocardiography
Hemodynamics
Biopsy
Light
Weights and Measures
Enzymes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Jeevanandam, V., Auteri, J. S., Sanchez, J. A., Barr, M. L., Ott, G. Y., Hsu, D. T., ... Rose, E. A. (1991). Improved heart preservation with University of Wisconsin solution: experimental and preliminary human experience. Circulation, 84(5 Suppl).

Improved heart preservation with University of Wisconsin solution : experimental and preliminary human experience. / Jeevanandam, V.; Auteri, J. S.; Sanchez, J. A.; Barr, M. L.; Ott, G. Y.; Hsu, Daphne T.; Marboe, C.; Smith, C. R.; Rose, E. A.

In: Circulation, Vol. 84, No. 5 Suppl, 11.1991.

Research output: Contribution to journalArticle

Jeevanandam, V, Auteri, JS, Sanchez, JA, Barr, ML, Ott, GY, Hsu, DT, Marboe, C, Smith, CR & Rose, EA 1991, 'Improved heart preservation with University of Wisconsin solution: experimental and preliminary human experience.', Circulation, vol. 84, no. 5 Suppl.
Jeevanandam, V. ; Auteri, J. S. ; Sanchez, J. A. ; Barr, M. L. ; Ott, G. Y. ; Hsu, Daphne T. ; Marboe, C. ; Smith, C. R. ; Rose, E. A. / Improved heart preservation with University of Wisconsin solution : experimental and preliminary human experience. In: Circulation. 1991 ; Vol. 84, No. 5 Suppl.
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