Improved detection of aneuploidy in malignant melanoma using multiparameter flow cytometry for s100 protein and DNA content

Hideko Kamino, Howard Ratech

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

DNA aneuploidy has been demonstrated to be an independent parameter of prognostic significance in malignant melanomas. In order to improve the detection of DNA aneuploidy in malignant melanomas, and to minimize diploid non-tumor cells in the sample, we developed a two-color staining strategy for S 100 protein and DNA content in paraffin embedded samples. The ability to detect aneuploidy, defined as DNA ploidy index ≤ 0.90 or ≥ 1.10, in 37 stage I malignant melanoma samples by flow cytometry analysis was significantly improved from 10.8% of cases using traditional one-color analysis for DNA content only (propidium iodide) to 32.4% of cases using two-color analysis for simultaneous measurement of both DNA (propidium iodide) and S100 protein (fluorescein conjugated antibody) (Chi-square with Yates' correction; p < 0.05). The largest increase in sensitivity was found in level I and II melanomas less than or equal to 0.76 mm in thickness. In addition, we report the new observation that multiple S100 protein-positive subpopulations were found significantly more frequently in malignant melanomas (23/37 cases) than in compound melanocytic nevi (5/22 cases) (Chi-square with Yates' correction; p < 0.01). These findings suggest that there is a previously unsuspected degree of tumor heterogeneity even in thin, presumably early, malignant melanomas.

Original languageEnglish (US)
Pages (from-to)392-396
Number of pages5
JournalJournal of Investigative Dermatology
Volume93
Issue number3
StatePublished - Sep 1989
Externally publishedYes

Fingerprint

Flow cytometry
S100 Proteins
Aneuploidy
Melanoma
Flow Cytometry
DNA
Propidium
Color
Pigmented Nevus
Ploidies
Fluorescein
Diploidy
Paraffin
Tumors
Cells
Staining and Labeling
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Dermatology

Cite this

Improved detection of aneuploidy in malignant melanoma using multiparameter flow cytometry for s100 protein and DNA content. / Kamino, Hideko; Ratech, Howard.

In: Journal of Investigative Dermatology, Vol. 93, No. 3, 09.1989, p. 392-396.

Research output: Contribution to journalArticle

@article{ebef3ed5804841ffb96ed22e82a97224,
title = "Improved detection of aneuploidy in malignant melanoma using multiparameter flow cytometry for s100 protein and DNA content",
abstract = "DNA aneuploidy has been demonstrated to be an independent parameter of prognostic significance in malignant melanomas. In order to improve the detection of DNA aneuploidy in malignant melanomas, and to minimize diploid non-tumor cells in the sample, we developed a two-color staining strategy for S 100 protein and DNA content in paraffin embedded samples. The ability to detect aneuploidy, defined as DNA ploidy index ≤ 0.90 or ≥ 1.10, in 37 stage I malignant melanoma samples by flow cytometry analysis was significantly improved from 10.8{\%} of cases using traditional one-color analysis for DNA content only (propidium iodide) to 32.4{\%} of cases using two-color analysis for simultaneous measurement of both DNA (propidium iodide) and S100 protein (fluorescein conjugated antibody) (Chi-square with Yates' correction; p < 0.05). The largest increase in sensitivity was found in level I and II melanomas less than or equal to 0.76 mm in thickness. In addition, we report the new observation that multiple S100 protein-positive subpopulations were found significantly more frequently in malignant melanomas (23/37 cases) than in compound melanocytic nevi (5/22 cases) (Chi-square with Yates' correction; p < 0.01). These findings suggest that there is a previously unsuspected degree of tumor heterogeneity even in thin, presumably early, malignant melanomas.",
author = "Hideko Kamino and Howard Ratech",
year = "1989",
month = "9",
language = "English (US)",
volume = "93",
pages = "392--396",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Improved detection of aneuploidy in malignant melanoma using multiparameter flow cytometry for s100 protein and DNA content

AU - Kamino, Hideko

AU - Ratech, Howard

PY - 1989/9

Y1 - 1989/9

N2 - DNA aneuploidy has been demonstrated to be an independent parameter of prognostic significance in malignant melanomas. In order to improve the detection of DNA aneuploidy in malignant melanomas, and to minimize diploid non-tumor cells in the sample, we developed a two-color staining strategy for S 100 protein and DNA content in paraffin embedded samples. The ability to detect aneuploidy, defined as DNA ploidy index ≤ 0.90 or ≥ 1.10, in 37 stage I malignant melanoma samples by flow cytometry analysis was significantly improved from 10.8% of cases using traditional one-color analysis for DNA content only (propidium iodide) to 32.4% of cases using two-color analysis for simultaneous measurement of both DNA (propidium iodide) and S100 protein (fluorescein conjugated antibody) (Chi-square with Yates' correction; p < 0.05). The largest increase in sensitivity was found in level I and II melanomas less than or equal to 0.76 mm in thickness. In addition, we report the new observation that multiple S100 protein-positive subpopulations were found significantly more frequently in malignant melanomas (23/37 cases) than in compound melanocytic nevi (5/22 cases) (Chi-square with Yates' correction; p < 0.01). These findings suggest that there is a previously unsuspected degree of tumor heterogeneity even in thin, presumably early, malignant melanomas.

AB - DNA aneuploidy has been demonstrated to be an independent parameter of prognostic significance in malignant melanomas. In order to improve the detection of DNA aneuploidy in malignant melanomas, and to minimize diploid non-tumor cells in the sample, we developed a two-color staining strategy for S 100 protein and DNA content in paraffin embedded samples. The ability to detect aneuploidy, defined as DNA ploidy index ≤ 0.90 or ≥ 1.10, in 37 stage I malignant melanoma samples by flow cytometry analysis was significantly improved from 10.8% of cases using traditional one-color analysis for DNA content only (propidium iodide) to 32.4% of cases using two-color analysis for simultaneous measurement of both DNA (propidium iodide) and S100 protein (fluorescein conjugated antibody) (Chi-square with Yates' correction; p < 0.05). The largest increase in sensitivity was found in level I and II melanomas less than or equal to 0.76 mm in thickness. In addition, we report the new observation that multiple S100 protein-positive subpopulations were found significantly more frequently in malignant melanomas (23/37 cases) than in compound melanocytic nevi (5/22 cases) (Chi-square with Yates' correction; p < 0.01). These findings suggest that there is a previously unsuspected degree of tumor heterogeneity even in thin, presumably early, malignant melanomas.

UR - http://www.scopus.com/inward/record.url?scp=0024452075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024452075&partnerID=8YFLogxK

M3 - Article

C2 - 2768839

AN - SCOPUS:0024452075

VL - 93

SP - 392

EP - 396

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 3

ER -