Impact of heme oxygenase-1 on cholesterol synthesis, cholesterol efflux and oxysterol formation in cultured astroglia

Jacob R. Hascalovici, Wei Song, Jacob Vaya, Soliman Khatib, Bianca Fuhrman, Michael Aviram, Hyman M. Schipper

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Up-regulation of heme oxygenase-1 (HO-1) and altered cholesterol (CH) metabolism are characteristic of Alzheimer-diseased neural tissues. The liver X receptor (LXR) is a molecular sensor of CH homeostasis. In the current study, we determined the effects of HO-1 over-expression and its byproducts iron (Fe 2+), carbon monoxide (CO) and bilirubin on CH biosynthesis, CH efflux and oxysterol formation in cultured astroglia. HO-1/LXR interactions were also investigated in the context of CH efflux. hHO-1 over-expression for 3 days (∼2-3-fold increase) resulted in a 30% increase in CH biosynthesis and a two-fold rise in CH efflux. Both effects were abrogated by the competitive HO inhibitor, tin mesoporphyrin. CO, released from administered CORM-3, significantly enhanced CH biosynthesis; a combination of CO and iron stimulated CH efflux. Free iron increased oxysterol formation three-fold. Co-treatment with LXR antagonists implicated LXR activation in the modulation of CH homeostasis by heme degradation products. In Alzheimer's disease and other neuropathological states, glial HO-1 induction may transduce ambient noxious stimuli (e.g. β-amyloid) into altered patterns of glial CH homeostasis. As the latter may impact synaptic plasticity and neuronal repair, modulation of glial HO-1 expression (by pharmacological or other means) may confer neuroprotection in patients with degenerative brain disorders.

Original languageEnglish (US)
Pages (from-to)72-81
Number of pages10
JournalJournal of Neurochemistry
Volume108
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Cholesterol
  • Heme oxygenase-1
  • LXR
  • Oxidative stress
  • Oxysterols

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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