Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women

Mimi Ghosh, Jason Daniels, Maria Pyra, Monika Juzumaite, Mariel Jais, Kerry J. Murphy, Tonya N. Taylor, Seble Kassaye, Lorie Benning, Mardge Cohen, Kathleen Weber

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sexual violence is associated with increased risk of HIV acquisition/transmission in women. Forced sex can result in physical trauma to the reproductive tract as well as severe psychological distress. However, immuno-biological mechanisms linking sexual violence and HIV susceptibility are incompletely understood. Using the Women's Interagency HIV Study repository, a total of 77 women were selected to form 4 groups, stratified by HIV serostatus, in the following categories: 1) no sexual abuse history and low depressive symptom score (below clinically significant cut-off, scores >16) (Control); 2) no sexual abuse history but high depressive symptom score, 16 (Depression); 3) chronic sexual abuse exposure and low depressive symptom score (Abuse); 4) chronic sexual abuse exposure and high depressive symptom score (Abuse+Depression). Inflammation-associated cytokines/chemokines/proteases (TNF-α, IL-6, IL-1α, IL-1β, TGF-β MIP-3α, IP-10, MCP-1, Cathepsin B), anti-inflammatory/ anti-HIV mediators (Secretory leukocyte protease inhibitor (SLPI), Elafin, beta defensin 2 (HBD2), alpha defensins (HNP 1-3), Thrombospondin (TSP-1), Serpin A1, A5, Cystatin A, B), and wound-healing mediators (Gro-α, VEGF, PDGF, EGF, FGF, IGF), were measured in cervical-vaginal lavage (CVL) using ELISA. Linear regression was used to model association of biomarkers with depression and abuse as predictor variables; the interaction between depression and abuse was also tested. Anti-HIV activity in CVL was tested using TZM-bl indicator cell line. In HIV-uninfected women, median levels of IL-6 (p = 0.04), IL-1α (p>0.01), TGF-β (p = 0.01), IP-10 (p = >0.01), PDGF (p>0.01) and FGF (p>0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased IL-1α (p>0.01), MIP-3α (p = 0.04), IP-10 (p>0.01), Serpin B1 (p = 0.01), FGF (p = 0.04) and decreased TGF-β (p>0.01), MCP-1 (p = 0.02), PDGF (p>0.01). Further, there was evidence of significant interactions between chronic sexual abuse and current depression for IL-1α, IP-10, Serpin A1, Cystatin B, and FGF. In HIVinfected women, median levels of TNF-α (p>0.01), IL-6 (p = 0.05), MIP-3α (p>0.01), and MCP-1 (p = 0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased MCP-1 (p = 0.03), Gro-α (p = 0.01) and decreased TNF-α (p>0.01), IL-1α (p = 0.02), MIP-3α (p>0.01) and Cathepsin B (p = 0.03). Current depressive symptoms were associated with significantly decreased MIP-3α (p>0.01). There was evidence of significant interactions between chronic sexual abuse and current depression for MCP-1 and FGF. No significant differences were observed in anti- HIV activity among all eight groups. Heat-map analyses revealed distinct immune network patterns, particularly in the Abuse groups for both HIV-infected and uninfected women. Our data indicates a complex relationship between chronic sexual abuse exposure, depressive symptoms, and FRT immune mediators that are also affected by HIV status. Association of chronic sexual abuse with increase in inflammation-associated cytokine/chemokine expression, along with impaired wound-healing associated growth-factors can create a microenvironment that can facilitate HIV infection. Evaluation of longitudinal changes in exposures and biomarkers are needed to untangle the immuno-biological mechanisms that may put women who endure life-long sexual abuse at increased risk for HIV.

Original languageEnglish (US)
Article numbere0198412
JournalPLoS One
Volume13
Issue number6
DOIs
StatePublished - Jun 1 2018

Fingerprint

sexual abuse
Sex Offenses
tissue repair
Interleukin-1
Wound Healing
inflammation
HIV
Depression
Inflammation
interleukin-1
1-methylcyclopropene
Interleukin-6
Cathepsin B
alpha 1-Antitrypsin
Association reactions
Biomarkers
signs and symptoms (animals and humans)
Chemokines
Elafin
Cystatin B

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women. / Ghosh, Mimi; Daniels, Jason; Pyra, Maria; Juzumaite, Monika; Jais, Mariel; Murphy, Kerry J.; Taylor, Tonya N.; Kassaye, Seble; Benning, Lorie; Cohen, Mardge; Weber, Kathleen.

In: PLoS One, Vol. 13, No. 6, e0198412, 01.06.2018.

Research output: Contribution to journalArticle

Ghosh, M, Daniels, J, Pyra, M, Juzumaite, M, Jais, M, Murphy, KJ, Taylor, TN, Kassaye, S, Benning, L, Cohen, M & Weber, K 2018, 'Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women', PLoS One, vol. 13, no. 6, e0198412. https://doi.org/10.1371/journal.pone.0198412
Ghosh, Mimi ; Daniels, Jason ; Pyra, Maria ; Juzumaite, Monika ; Jais, Mariel ; Murphy, Kerry J. ; Taylor, Tonya N. ; Kassaye, Seble ; Benning, Lorie ; Cohen, Mardge ; Weber, Kathleen. / Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women. In: PLoS One. 2018 ; Vol. 13, No. 6.
@article{4258d4af83d04e98abd870063e1716b1,
title = "Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women",
abstract = "Sexual violence is associated with increased risk of HIV acquisition/transmission in women. Forced sex can result in physical trauma to the reproductive tract as well as severe psychological distress. However, immuno-biological mechanisms linking sexual violence and HIV susceptibility are incompletely understood. Using the Women's Interagency HIV Study repository, a total of 77 women were selected to form 4 groups, stratified by HIV serostatus, in the following categories: 1) no sexual abuse history and low depressive symptom score (below clinically significant cut-off, scores >16) (Control); 2) no sexual abuse history but high depressive symptom score, 16 (Depression); 3) chronic sexual abuse exposure and low depressive symptom score (Abuse); 4) chronic sexual abuse exposure and high depressive symptom score (Abuse+Depression). Inflammation-associated cytokines/chemokines/proteases (TNF-α, IL-6, IL-1α, IL-1β, TGF-β MIP-3α, IP-10, MCP-1, Cathepsin B), anti-inflammatory/ anti-HIV mediators (Secretory leukocyte protease inhibitor (SLPI), Elafin, beta defensin 2 (HBD2), alpha defensins (HNP 1-3), Thrombospondin (TSP-1), Serpin A1, A5, Cystatin A, B), and wound-healing mediators (Gro-α, VEGF, PDGF, EGF, FGF, IGF), were measured in cervical-vaginal lavage (CVL) using ELISA. Linear regression was used to model association of biomarkers with depression and abuse as predictor variables; the interaction between depression and abuse was also tested. Anti-HIV activity in CVL was tested using TZM-bl indicator cell line. In HIV-uninfected women, median levels of IL-6 (p = 0.04), IL-1α (p>0.01), TGF-β (p = 0.01), IP-10 (p = >0.01), PDGF (p>0.01) and FGF (p>0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased IL-1α (p>0.01), MIP-3α (p = 0.04), IP-10 (p>0.01), Serpin B1 (p = 0.01), FGF (p = 0.04) and decreased TGF-β (p>0.01), MCP-1 (p = 0.02), PDGF (p>0.01). Further, there was evidence of significant interactions between chronic sexual abuse and current depression for IL-1α, IP-10, Serpin A1, Cystatin B, and FGF. In HIVinfected women, median levels of TNF-α (p>0.01), IL-6 (p = 0.05), MIP-3α (p>0.01), and MCP-1 (p = 0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased MCP-1 (p = 0.03), Gro-α (p = 0.01) and decreased TNF-α (p>0.01), IL-1α (p = 0.02), MIP-3α (p>0.01) and Cathepsin B (p = 0.03). Current depressive symptoms were associated with significantly decreased MIP-3α (p>0.01). There was evidence of significant interactions between chronic sexual abuse and current depression for MCP-1 and FGF. No significant differences were observed in anti- HIV activity among all eight groups. Heat-map analyses revealed distinct immune network patterns, particularly in the Abuse groups for both HIV-infected and uninfected women. Our data indicates a complex relationship between chronic sexual abuse exposure, depressive symptoms, and FRT immune mediators that are also affected by HIV status. Association of chronic sexual abuse with increase in inflammation-associated cytokine/chemokine expression, along with impaired wound-healing associated growth-factors can create a microenvironment that can facilitate HIV infection. Evaluation of longitudinal changes in exposures and biomarkers are needed to untangle the immuno-biological mechanisms that may put women who endure life-long sexual abuse at increased risk for HIV.",
author = "Mimi Ghosh and Jason Daniels and Maria Pyra and Monika Juzumaite and Mariel Jais and Murphy, {Kerry J.} and Taylor, {Tonya N.} and Seble Kassaye and Lorie Benning and Mardge Cohen and Kathleen Weber",
year = "2018",
month = "6",
day = "1",
doi = "10.1371/journal.pone.0198412",
language = "English (US)",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Impact of chronic sexual abuse and depression on inflammation and wound healing in the female reproductive tract of HIV-uninfected and HIV-infected women

AU - Ghosh, Mimi

AU - Daniels, Jason

AU - Pyra, Maria

AU - Juzumaite, Monika

AU - Jais, Mariel

AU - Murphy, Kerry J.

AU - Taylor, Tonya N.

AU - Kassaye, Seble

AU - Benning, Lorie

AU - Cohen, Mardge

AU - Weber, Kathleen

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Sexual violence is associated with increased risk of HIV acquisition/transmission in women. Forced sex can result in physical trauma to the reproductive tract as well as severe psychological distress. However, immuno-biological mechanisms linking sexual violence and HIV susceptibility are incompletely understood. Using the Women's Interagency HIV Study repository, a total of 77 women were selected to form 4 groups, stratified by HIV serostatus, in the following categories: 1) no sexual abuse history and low depressive symptom score (below clinically significant cut-off, scores >16) (Control); 2) no sexual abuse history but high depressive symptom score, 16 (Depression); 3) chronic sexual abuse exposure and low depressive symptom score (Abuse); 4) chronic sexual abuse exposure and high depressive symptom score (Abuse+Depression). Inflammation-associated cytokines/chemokines/proteases (TNF-α, IL-6, IL-1α, IL-1β, TGF-β MIP-3α, IP-10, MCP-1, Cathepsin B), anti-inflammatory/ anti-HIV mediators (Secretory leukocyte protease inhibitor (SLPI), Elafin, beta defensin 2 (HBD2), alpha defensins (HNP 1-3), Thrombospondin (TSP-1), Serpin A1, A5, Cystatin A, B), and wound-healing mediators (Gro-α, VEGF, PDGF, EGF, FGF, IGF), were measured in cervical-vaginal lavage (CVL) using ELISA. Linear regression was used to model association of biomarkers with depression and abuse as predictor variables; the interaction between depression and abuse was also tested. Anti-HIV activity in CVL was tested using TZM-bl indicator cell line. In HIV-uninfected women, median levels of IL-6 (p = 0.04), IL-1α (p>0.01), TGF-β (p = 0.01), IP-10 (p = >0.01), PDGF (p>0.01) and FGF (p>0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased IL-1α (p>0.01), MIP-3α (p = 0.04), IP-10 (p>0.01), Serpin B1 (p = 0.01), FGF (p = 0.04) and decreased TGF-β (p>0.01), MCP-1 (p = 0.02), PDGF (p>0.01). Further, there was evidence of significant interactions between chronic sexual abuse and current depression for IL-1α, IP-10, Serpin A1, Cystatin B, and FGF. In HIVinfected women, median levels of TNF-α (p>0.01), IL-6 (p = 0.05), MIP-3α (p>0.01), and MCP-1 (p = 0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased MCP-1 (p = 0.03), Gro-α (p = 0.01) and decreased TNF-α (p>0.01), IL-1α (p = 0.02), MIP-3α (p>0.01) and Cathepsin B (p = 0.03). Current depressive symptoms were associated with significantly decreased MIP-3α (p>0.01). There was evidence of significant interactions between chronic sexual abuse and current depression for MCP-1 and FGF. No significant differences were observed in anti- HIV activity among all eight groups. Heat-map analyses revealed distinct immune network patterns, particularly in the Abuse groups for both HIV-infected and uninfected women. Our data indicates a complex relationship between chronic sexual abuse exposure, depressive symptoms, and FRT immune mediators that are also affected by HIV status. Association of chronic sexual abuse with increase in inflammation-associated cytokine/chemokine expression, along with impaired wound-healing associated growth-factors can create a microenvironment that can facilitate HIV infection. Evaluation of longitudinal changes in exposures and biomarkers are needed to untangle the immuno-biological mechanisms that may put women who endure life-long sexual abuse at increased risk for HIV.

AB - Sexual violence is associated with increased risk of HIV acquisition/transmission in women. Forced sex can result in physical trauma to the reproductive tract as well as severe psychological distress. However, immuno-biological mechanisms linking sexual violence and HIV susceptibility are incompletely understood. Using the Women's Interagency HIV Study repository, a total of 77 women were selected to form 4 groups, stratified by HIV serostatus, in the following categories: 1) no sexual abuse history and low depressive symptom score (below clinically significant cut-off, scores >16) (Control); 2) no sexual abuse history but high depressive symptom score, 16 (Depression); 3) chronic sexual abuse exposure and low depressive symptom score (Abuse); 4) chronic sexual abuse exposure and high depressive symptom score (Abuse+Depression). Inflammation-associated cytokines/chemokines/proteases (TNF-α, IL-6, IL-1α, IL-1β, TGF-β MIP-3α, IP-10, MCP-1, Cathepsin B), anti-inflammatory/ anti-HIV mediators (Secretory leukocyte protease inhibitor (SLPI), Elafin, beta defensin 2 (HBD2), alpha defensins (HNP 1-3), Thrombospondin (TSP-1), Serpin A1, A5, Cystatin A, B), and wound-healing mediators (Gro-α, VEGF, PDGF, EGF, FGF, IGF), were measured in cervical-vaginal lavage (CVL) using ELISA. Linear regression was used to model association of biomarkers with depression and abuse as predictor variables; the interaction between depression and abuse was also tested. Anti-HIV activity in CVL was tested using TZM-bl indicator cell line. In HIV-uninfected women, median levels of IL-6 (p = 0.04), IL-1α (p>0.01), TGF-β (p = 0.01), IP-10 (p = >0.01), PDGF (p>0.01) and FGF (p>0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased IL-1α (p>0.01), MIP-3α (p = 0.04), IP-10 (p>0.01), Serpin B1 (p = 0.01), FGF (p = 0.04) and decreased TGF-β (p>0.01), MCP-1 (p = 0.02), PDGF (p>0.01). Further, there was evidence of significant interactions between chronic sexual abuse and current depression for IL-1α, IP-10, Serpin A1, Cystatin B, and FGF. In HIVinfected women, median levels of TNF-α (p>0.01), IL-6 (p = 0.05), MIP-3α (p>0.01), and MCP-1 (p = 0.01), differed significantly between groups. Specifically, an association was found between chronic sexual abuse and increased MCP-1 (p = 0.03), Gro-α (p = 0.01) and decreased TNF-α (p>0.01), IL-1α (p = 0.02), MIP-3α (p>0.01) and Cathepsin B (p = 0.03). Current depressive symptoms were associated with significantly decreased MIP-3α (p>0.01). There was evidence of significant interactions between chronic sexual abuse and current depression for MCP-1 and FGF. No significant differences were observed in anti- HIV activity among all eight groups. Heat-map analyses revealed distinct immune network patterns, particularly in the Abuse groups for both HIV-infected and uninfected women. Our data indicates a complex relationship between chronic sexual abuse exposure, depressive symptoms, and FRT immune mediators that are also affected by HIV status. Association of chronic sexual abuse with increase in inflammation-associated cytokine/chemokine expression, along with impaired wound-healing associated growth-factors can create a microenvironment that can facilitate HIV infection. Evaluation of longitudinal changes in exposures and biomarkers are needed to untangle the immuno-biological mechanisms that may put women who endure life-long sexual abuse at increased risk for HIV.

UR - http://www.scopus.com/inward/record.url?scp=85048435918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048435918&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0198412

DO - 10.1371/journal.pone.0198412

M3 - Article

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e0198412

ER -