TY - JOUR
T1 - Impact of 6-month frozen storage of cervical specimens in alkaline buffer conditions on human papillomavirus genotyping
AU - LaMere, Brandon J.
AU - Howell, Renee
AU - Fetterman, Barbara
AU - Shieh, Jen
AU - Castle, Philip E.
N1 - Funding Information:
This research was supported by the Intramural Research Program of the NIH, NCI and by Kaiser Permanente Northern California (KPNC).
PY - 2008/8
Y1 - 2008/8
N2 - The impact of 6-month storage of cervical specimens under alkaline conditions that occurs as the result of Hybrid Capture 2 testing on human papillomavirus (HPV) genotyping is not well documented. To examine this issue, 143 frozen hc2-positive specimens in specimen transport medium were selected at random from each of the following groups: specimens stored for 6 months, 4 months, and 2.5 months under alkaline pH (pH 12-13) and specimens stored 1 month at neutral pH (pH 6-7) as controls. Specimens were tested in a masked fashion for 20 HPV genotypes (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) using a prototype, research-use-only GP5+/6+ L1 consensus PCR method and multiplex hybridization using Luminex® xMAP® for detection of specific HPV genotypes One control specimen had missing test results. There were no statistical differences in the number of HPV genotypes detected, number of carcinogenic HPV genotypes detected, or in the signal strength among HPV-positive results across groups. Six-month frozen storage of cervical specimens at alkaline pH had little impact on testing for HPV genotypes among hc2-positive women using this HPV genotyping method.
AB - The impact of 6-month storage of cervical specimens under alkaline conditions that occurs as the result of Hybrid Capture 2 testing on human papillomavirus (HPV) genotyping is not well documented. To examine this issue, 143 frozen hc2-positive specimens in specimen transport medium were selected at random from each of the following groups: specimens stored for 6 months, 4 months, and 2.5 months under alkaline pH (pH 12-13) and specimens stored 1 month at neutral pH (pH 6-7) as controls. Specimens were tested in a masked fashion for 20 HPV genotypes (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) using a prototype, research-use-only GP5+/6+ L1 consensus PCR method and multiplex hybridization using Luminex® xMAP® for detection of specific HPV genotypes One control specimen had missing test results. There were no statistical differences in the number of HPV genotypes detected, number of carcinogenic HPV genotypes detected, or in the signal strength among HPV-positive results across groups. Six-month frozen storage of cervical specimens at alkaline pH had little impact on testing for HPV genotypes among hc2-positive women using this HPV genotyping method.
KW - HPV genotypes
KW - Human papillomavirus (HPV)
KW - PCR
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U2 - 10.1016/j.jviromet.2008.05.017
DO - 10.1016/j.jviromet.2008.05.017
M3 - Article
C2 - 18571737
AN - SCOPUS:46949093337
SN - 0166-0934
VL - 151
SP - 298
EP - 300
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 2
ER -