Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma

Susan A. Fineberg, E. Marsh, F. Alfonso, E. Espiritu, S. R S Gottesman, E. Morosi, H. D. Feiner

Research output: Contribution to journalArticle

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Abstract

Immunophenotypic evaluations of the bone marrow (BM) are reported on 69 aspirates from 58 patients who had non-Hodgkin's lymphoma or chronic lymphocytic leukemia involving the BM. Using flow cytometry and immunofluorescence microscopy on density gradient isolated BM mononuclear cells, the neoplasm could be identified and characterized in 59 aspirates (86%) from 49 patients (84%). Using International Working Formulation guidelines the neoplasms were classified on the basis of prior or subsequent histopathology of lymph node, spleen, skin, or other soft tissue site, or by evaluation of peripheral blood in chronic lymphocytic leukemia. In nine cases the lymphoma could not be completely classified according to International Working Formulation guidelines because only BM was available for evaluation. The neoplasm in the BM was identified and characterized immunophenotypically in all 29 cases of chronic lymphocytic leukemia/well-differentiated lymphocytic lymphoma (WDLL) (100%), in 11 of 12 cases of low-grade lymphoma other than WDLL (92%), in 11 of 15 cases of intermediate-grade lymphoma (73%), and in two of four cases of high-grade lymphoma (50%). Six of the nine cases not classified by International Working Formulation guidelines could be characterized immunophenotypically. In 10 cases immunophenotypic studies revealed negative findings, although the concurrent core biopsy specimens were positive. In two cases immunophenotypic studies with positive findings accompanied a negative core biopsy specimen. A panel of immunohistochemical reagents reactive with fixative/paraffin-resistant antigens was used for a retrospective evaluation of the 69 core biopsy specimens. When compared with the immunophenotypic data obtained from the marrow aspirates these results proved to be only moderately reliable in B-lineage neoplasmas and unreliable in T-cell neoplasms. Thus, immunophenotyping of aspirated marrow by flow cytometry was found to be the most reliable method for determining the antigenic profiles of BM-based lymphomas.

Original languageEnglish (US)
Pages (from-to)636-642
Number of pages7
JournalHuman Pathology
Volume24
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Non-Hodgkin's Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Bone Marrow
Guidelines
Biopsy
Bone Marrow Neoplasms
Lymphoma
Flow Cytometry
Immunophenotyping
Neoplasms
Fixatives
Fluorescence Microscopy
Bone Marrow Cells
Paraffin
Spleen
Lymph Nodes
T-Lymphocytes
Antigens
Skin

Keywords

  • bone marrow
  • flow cytometry
  • immunohistochemistry
  • immunophenotype
  • non-Hodgkin's lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fineberg, S. A., Marsh, E., Alfonso, F., Espiritu, E., Gottesman, S. R. S., Morosi, E., & Feiner, H. D. (1993). Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma. Human Pathology, 24(6), 636-642. https://doi.org/10.1016/0046-8177(93)90244-B

Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma. / Fineberg, Susan A.; Marsh, E.; Alfonso, F.; Espiritu, E.; Gottesman, S. R S; Morosi, E.; Feiner, H. D.

In: Human Pathology, Vol. 24, No. 6, 1993, p. 636-642.

Research output: Contribution to journalArticle

Fineberg, SA, Marsh, E, Alfonso, F, Espiritu, E, Gottesman, SRS, Morosi, E & Feiner, HD 1993, 'Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma', Human Pathology, vol. 24, no. 6, pp. 636-642. https://doi.org/10.1016/0046-8177(93)90244-B
Fineberg SA, Marsh E, Alfonso F, Espiritu E, Gottesman SRS, Morosi E et al. Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma. Human Pathology. 1993;24(6):636-642. https://doi.org/10.1016/0046-8177(93)90244-B
Fineberg, Susan A. ; Marsh, E. ; Alfonso, F. ; Espiritu, E. ; Gottesman, S. R S ; Morosi, E. ; Feiner, H. D. / Immunophenotypic evaluation of the Bone Marrow in non-Hodgkin's lymphoma. In: Human Pathology. 1993 ; Vol. 24, No. 6. pp. 636-642.
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