Immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes

Chang Hui Zhou, Yi Tian, Bo Yang, Xiang Hu, Hong Liang Jiao, Yun Fan Zhou, Cheng Chun Wang, Chen Xi Gu, Ning Jing Lei, Fangxia Guan

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect, but there are seldom reports concerning the immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims. OBJECTIVE: To investigate the immunomodulatory effects and mechanisms of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes. METHODS: Mesenchymal stem cells were isolateded from Wharton's jelly of human umbilical cord by tissue culture. T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton's jelly-derived mesenchymal stem cells and umbilical cord Wharton's jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader. Expression of cytokines including transforming growth factor-beta 1 (TGF-β1) and interferon-γ (IFN-γ) was evaluated by enzyme-labeled immunosorbent assay. RESULTS AND CONCLUSION: Wharton's jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin. The proliferation inhibition rate was 56% (P < 0.01). Wharton's jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin, in a dose-dependent fashion. The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton's jelly-derived mesenchymal stem cells supernatant and 100% Wharton's jelly-derived mesenchymal stem cells supernatant groups (P < 0.05). Wharton's jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes (P < 0.05). The secretion of TGF-β1 was lower in the coculture of Wharton's jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton's jelly-derived mesenchymal stem cells alone group (P < 0.05). These indicated that Wharton's jelly-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin. The mechanims may be associated with cell contant and inhibition of γ-interferon secrted from T-lymphocytes.

Original languageEnglish (US)
Pages (from-to)2485-2491
Number of pages7
JournalJournal of Clinical Rehabilitative Tissue Engineering Research
Volume14
Issue number14
DOIs
StatePublished - Apr 2 2010
Externally publishedYes

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Wharton Jelly
T-cells
Umbilical Cord
Stem cells
Mesenchymal Stromal Cells
Blood
T-Lymphocytes
Phytohemagglutinins
Interferons
Coculture Techniques
Transforming Growth Factor beta
Tissue culture
Immunosorbents

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biomedical Engineering
  • Transplantation

Cite this

Immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes. / Zhou, Chang Hui; Tian, Yi; Yang, Bo; Hu, Xiang; Jiao, Hong Liang; Zhou, Yun Fan; Wang, Cheng Chun; Gu, Chen Xi; Lei, Ning Jing; Guan, Fangxia.

In: Journal of Clinical Rehabilitative Tissue Engineering Research, Vol. 14, No. 14, 02.04.2010, p. 2485-2491.

Research output: Contribution to journalArticle

Zhou, Chang Hui ; Tian, Yi ; Yang, Bo ; Hu, Xiang ; Jiao, Hong Liang ; Zhou, Yun Fan ; Wang, Cheng Chun ; Gu, Chen Xi ; Lei, Ning Jing ; Guan, Fangxia. / Immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes. In: Journal of Clinical Rehabilitative Tissue Engineering Research. 2010 ; Vol. 14, No. 14. pp. 2485-2491.
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abstract = "BACKGROUND: Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect, but there are seldom reports concerning the immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims. OBJECTIVE: To investigate the immunomodulatory effects and mechanisms of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes. METHODS: Mesenchymal stem cells were isolateded from Wharton's jelly of human umbilical cord by tissue culture. T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton's jelly-derived mesenchymal stem cells and umbilical cord Wharton's jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader. Expression of cytokines including transforming growth factor-beta 1 (TGF-β1) and interferon-γ (IFN-γ) was evaluated by enzyme-labeled immunosorbent assay. RESULTS AND CONCLUSION: Wharton's jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin. The proliferation inhibition rate was 56{\%} (P < 0.01). Wharton's jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin, in a dose-dependent fashion. The proliferation inhibition rates were 8.3{\%} and 27{\%} respectively in the 50{\%} Wharton's jelly-derived mesenchymal stem cells supernatant and 100{\%} Wharton's jelly-derived mesenchymal stem cells supernatant groups (P < 0.05). Wharton's jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes (P < 0.05). The secretion of TGF-β1 was lower in the coculture of Wharton's jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton's jelly-derived mesenchymal stem cells alone group (P < 0.05). These indicated that Wharton's jelly-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin. The mechanims may be associated with cell contant and inhibition of γ-interferon secrted from T-lymphocytes.",
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T1 - Immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells from human umbilical cord on human peripheral blood T lymphocytes

AU - Zhou, Chang Hui

AU - Tian, Yi

AU - Yang, Bo

AU - Hu, Xiang

AU - Jiao, Hong Liang

AU - Zhou, Yun Fan

AU - Wang, Cheng Chun

AU - Gu, Chen Xi

AU - Lei, Ning Jing

AU - Guan, Fangxia

PY - 2010/4/2

Y1 - 2010/4/2

N2 - BACKGROUND: Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect, but there are seldom reports concerning the immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims. OBJECTIVE: To investigate the immunomodulatory effects and mechanisms of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes. METHODS: Mesenchymal stem cells were isolateded from Wharton's jelly of human umbilical cord by tissue culture. T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton's jelly-derived mesenchymal stem cells and umbilical cord Wharton's jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader. Expression of cytokines including transforming growth factor-beta 1 (TGF-β1) and interferon-γ (IFN-γ) was evaluated by enzyme-labeled immunosorbent assay. RESULTS AND CONCLUSION: Wharton's jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin. The proliferation inhibition rate was 56% (P < 0.01). Wharton's jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin, in a dose-dependent fashion. The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton's jelly-derived mesenchymal stem cells supernatant and 100% Wharton's jelly-derived mesenchymal stem cells supernatant groups (P < 0.05). Wharton's jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes (P < 0.05). The secretion of TGF-β1 was lower in the coculture of Wharton's jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton's jelly-derived mesenchymal stem cells alone group (P < 0.05). These indicated that Wharton's jelly-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin. The mechanims may be associated with cell contant and inhibition of γ-interferon secrted from T-lymphocytes.

AB - BACKGROUND: Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect, but there are seldom reports concerning the immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims. OBJECTIVE: To investigate the immunomodulatory effects and mechanisms of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes. METHODS: Mesenchymal stem cells were isolateded from Wharton's jelly of human umbilical cord by tissue culture. T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton's jelly-derived mesenchymal stem cells and umbilical cord Wharton's jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader. Expression of cytokines including transforming growth factor-beta 1 (TGF-β1) and interferon-γ (IFN-γ) was evaluated by enzyme-labeled immunosorbent assay. RESULTS AND CONCLUSION: Wharton's jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin. The proliferation inhibition rate was 56% (P < 0.01). Wharton's jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin, in a dose-dependent fashion. The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton's jelly-derived mesenchymal stem cells supernatant and 100% Wharton's jelly-derived mesenchymal stem cells supernatant groups (P < 0.05). Wharton's jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes (P < 0.05). The secretion of TGF-β1 was lower in the coculture of Wharton's jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton's jelly-derived mesenchymal stem cells alone group (P < 0.05). These indicated that Wharton's jelly-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin. The mechanims may be associated with cell contant and inhibition of γ-interferon secrted from T-lymphocytes.

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