Immunologic responses of HIV-1-infected study subjects to immunization with a mixture of peptide protein derivative-V3 loop peptide conjugates

Arye Rubinstein, Yaffa Mizrachi, Massimo Pettoello-Mantovani, Jack Lenz, Geng Qi Liu, Yair Rubinstein, Harris Goldstein, Israel Yust, Michael Burke, Nurit Vardinon, Zvi Spirer, Stanley J. Cryz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

V3 loop peptide sequences from several HIV-1 strains were covalently linked to purified protein derivative (PPD) of Mycobacterium tuberculosis. A mixture: of PPD conjugates of V3 loop peptides from six different strains of HIV-1 induced a stronger antibody response than a single V3 peptide-conjugate administered to guinea pigs and humans. Sera from animals immunized with a PPD-six peptide-PPD conjugate neutralized multiple primary-isolate strains of HIV-1. Potent immune responses were noted only when animals were primed with bacillus Calmette-Guerin (BCG), PPD was covalently bound to the peptides, and PPD was used as the carrier protein. Based on these animal studies, an immunogen consisting of PPD-conjugated V3 loop peptides from five HIV-1 strains was tested in 7 HIV-1 seropositive PPD skin test-positive study subjects. Vaccinees exhibited over time a uniform increase in neutralizing antibodies for both laboratory adapted and primary isolates of HIV-1 including strains from multiple clades. In 3 patients with baseline viral loads between 8000 and 12,000 RNA copies/ml, the viral load declined in 2 patients to <400 copies/ml and in 1 patient to 1200 copies/ml without concurrent administration of highly active antiretroviral therapy (HAART).

Original languageEnglish (US)
Pages (from-to)467-476
Number of pages10
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume22
Issue number5
StatePublished - Dec 15 1999

Fingerprint

HIV-1
Immunization
Peptides
Proteins
Viral Load
Highly Active Antiretroviral Therapy
Mycobacterium bovis
Skin Tests
Neutralizing Antibodies
Mycobacterium tuberculosis
Antibody Formation
Carrier Proteins
Guinea Pigs
RNA
Serum

Keywords

  • Neutralizing antibodies
  • PPD-V3 loop conjugates
  • Primary neutralizing domain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

Cite this

Immunologic responses of HIV-1-infected study subjects to immunization with a mixture of peptide protein derivative-V3 loop peptide conjugates. / Rubinstein, Arye; Mizrachi, Yaffa; Pettoello-Mantovani, Massimo; Lenz, Jack; Liu, Geng Qi; Rubinstein, Yair; Goldstein, Harris; Yust, Israel; Burke, Michael; Vardinon, Nurit; Spirer, Zvi; Cryz, Stanley J.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 22, No. 5, 15.12.1999, p. 467-476.

Research output: Contribution to journalArticle

Rubinstein, Arye ; Mizrachi, Yaffa ; Pettoello-Mantovani, Massimo ; Lenz, Jack ; Liu, Geng Qi ; Rubinstein, Yair ; Goldstein, Harris ; Yust, Israel ; Burke, Michael ; Vardinon, Nurit ; Spirer, Zvi ; Cryz, Stanley J. / Immunologic responses of HIV-1-infected study subjects to immunization with a mixture of peptide protein derivative-V3 loop peptide conjugates. In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1999 ; Vol. 22, No. 5. pp. 467-476.
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