Immunolocalization of estrogen receptor α and β in human fetal prostate

Ellen Shapiro, Hongying Huang, Rachel J. Masch, Deborah E. McFadden, E. Lynette Wilson, Xue Ru Wu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Purpose: We examined the immunolocalization of estrogen receptor (ER)α and ERβ in the human fetal prostate. Materials and Methods: Tissue sections from human fetal prostates at 7 to 22 weeks of gestation were stained with antibodies to ERα, ERβ, and cytokeratin 10 and 14. Results: ERα expression was not detected until 15 weeks of gestation with sparse staining in the utricle. By 19 weeks increased ERα expression was seen in the luminal cells of the ventral urogenital epithelium (UGE), basal cells of the dorsal UGE, utricle, distal periurethral ducts, peripheral stroma and posterior prostatic duct. K14 was detected in basal cells of the UGE and in several posterior acini. At 22 weeks ERα expression was more intense in all of these areas. ERβ was expressed throughout the UGE, ejaculatory ducts, mullerian ducts and entire stroma at 7 weeks. Intense ERβ staining was observed in these areas and in the prostatic buds by 8 weeks with persistent intense staining through 22 weeks. Conclusions: To our knowledge we report the first immunolocalization of ERα in the human fetal prostate and the earliest demonstration of ERβ expression in the prostate at 7 weeks of gestation. ERβ expression is intense during ductal morphogenesis, suggesting a role in normal glandular growth and proliferation. The induction of squamous metaplasia in the UGE, distal periurethral ducts and utricle is associated with ERα expression in these areas, while the induction of squamous metaplasia in peripheral prostatic acini is associated with peripheral stromal ERα expression. This study suggests estrogen signaling pathways in the human fetal prostate via ERα that involve epithelial-epithelial and epithelial-stromal interactions.

Original languageEnglish (US)
Pages (from-to)2051-2053
Number of pages3
JournalJournal of Urology
Volume174
Issue number5
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Estrogen Receptors
Prostate
Saccule and Utricle
Epithelium
Metaplasia
Staining and Labeling
Pregnancy
Ejaculatory Ducts
Keratin-14
Mullerian Ducts
Morphogenesis
Estrogens

Keywords

  • Estrogen receptor alpha
  • Estrogen receptor beta
  • Fetus
  • Prostate

ASJC Scopus subject areas

  • Urology

Cite this

Shapiro, E., Huang, H., Masch, R. J., McFadden, D. E., Wilson, E. L., & Wu, X. R. (2005). Immunolocalization of estrogen receptor α and β in human fetal prostate. Journal of Urology, 174(5), 2051-2053. https://doi.org/10.1097/01.ju.0000176472.90432.5b

Immunolocalization of estrogen receptor α and β in human fetal prostate. / Shapiro, Ellen; Huang, Hongying; Masch, Rachel J.; McFadden, Deborah E.; Wilson, E. Lynette; Wu, Xue Ru.

In: Journal of Urology, Vol. 174, No. 5, 11.2005, p. 2051-2053.

Research output: Contribution to journalArticle

Shapiro, E, Huang, H, Masch, RJ, McFadden, DE, Wilson, EL & Wu, XR 2005, 'Immunolocalization of estrogen receptor α and β in human fetal prostate', Journal of Urology, vol. 174, no. 5, pp. 2051-2053. https://doi.org/10.1097/01.ju.0000176472.90432.5b
Shapiro, Ellen ; Huang, Hongying ; Masch, Rachel J. ; McFadden, Deborah E. ; Wilson, E. Lynette ; Wu, Xue Ru. / Immunolocalization of estrogen receptor α and β in human fetal prostate. In: Journal of Urology. 2005 ; Vol. 174, No. 5. pp. 2051-2053.
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abstract = "Purpose: We examined the immunolocalization of estrogen receptor (ER)α and ERβ in the human fetal prostate. Materials and Methods: Tissue sections from human fetal prostates at 7 to 22 weeks of gestation were stained with antibodies to ERα, ERβ, and cytokeratin 10 and 14. Results: ERα expression was not detected until 15 weeks of gestation with sparse staining in the utricle. By 19 weeks increased ERα expression was seen in the luminal cells of the ventral urogenital epithelium (UGE), basal cells of the dorsal UGE, utricle, distal periurethral ducts, peripheral stroma and posterior prostatic duct. K14 was detected in basal cells of the UGE and in several posterior acini. At 22 weeks ERα expression was more intense in all of these areas. ERβ was expressed throughout the UGE, ejaculatory ducts, mullerian ducts and entire stroma at 7 weeks. Intense ERβ staining was observed in these areas and in the prostatic buds by 8 weeks with persistent intense staining through 22 weeks. Conclusions: To our knowledge we report the first immunolocalization of ERα in the human fetal prostate and the earliest demonstration of ERβ expression in the prostate at 7 weeks of gestation. ERβ expression is intense during ductal morphogenesis, suggesting a role in normal glandular growth and proliferation. The induction of squamous metaplasia in the UGE, distal periurethral ducts and utricle is associated with ERα expression in these areas, while the induction of squamous metaplasia in peripheral prostatic acini is associated with peripheral stromal ERα expression. This study suggests estrogen signaling pathways in the human fetal prostate via ERα that involve epithelial-epithelial and epithelial-stromal interactions.",
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AU - Wu, Xue Ru

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AB - Purpose: We examined the immunolocalization of estrogen receptor (ER)α and ERβ in the human fetal prostate. Materials and Methods: Tissue sections from human fetal prostates at 7 to 22 weeks of gestation were stained with antibodies to ERα, ERβ, and cytokeratin 10 and 14. Results: ERα expression was not detected until 15 weeks of gestation with sparse staining in the utricle. By 19 weeks increased ERα expression was seen in the luminal cells of the ventral urogenital epithelium (UGE), basal cells of the dorsal UGE, utricle, distal periurethral ducts, peripheral stroma and posterior prostatic duct. K14 was detected in basal cells of the UGE and in several posterior acini. At 22 weeks ERα expression was more intense in all of these areas. ERβ was expressed throughout the UGE, ejaculatory ducts, mullerian ducts and entire stroma at 7 weeks. Intense ERβ staining was observed in these areas and in the prostatic buds by 8 weeks with persistent intense staining through 22 weeks. Conclusions: To our knowledge we report the first immunolocalization of ERα in the human fetal prostate and the earliest demonstration of ERβ expression in the prostate at 7 weeks of gestation. ERβ expression is intense during ductal morphogenesis, suggesting a role in normal glandular growth and proliferation. The induction of squamous metaplasia in the UGE, distal periurethral ducts and utricle is associated with ERα expression in these areas, while the induction of squamous metaplasia in peripheral prostatic acini is associated with peripheral stromal ERα expression. This study suggests estrogen signaling pathways in the human fetal prostate via ERα that involve epithelial-epithelial and epithelial-stromal interactions.

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