Immunolabeling of thin sections of Drosophila tissues for transmission electron microscopy

Kent L. McDonald, David J. Sharp, Wayne Rickoll

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The main advantage of electron microscopy (EM) for immunolabeling is resolution, but there is also another aspect that is often overlooked. For many investigators, the definitive image of an organelle is the one generated by EM. This is especially true for membranous organelles, with the possible exception of the nucleus and plant vacuoles. For example, references to the Golgi apparatus, smooth and rough endoplasmic reticulum, centriole, kinetochore, or mitochondrion typically bring to mind the images in an electron micrograph. The components of the cytoskeleton also have characteristic structural features that are associated with their EM image. Thus, it can be more effective for investigators to view gold particles superimposed over the image of a microtubule (MT) or mitochondrion using EM than to see bright dots or lines in the light microscope. This is especially true if the immunofluorescence image is of fixed cells. Here, we provide an overview of methods of EM immunolabeling used for localizing specific antigens on thin sections of Drosophila tissues.

Original languageEnglish (US)
Pages (from-to)838-841
Number of pages4
JournalCold Spring Harbor Protocols
Volume7
Issue number7
DOIs
StatePublished - Jul 2012
Externally publishedYes

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Transmission Electron Microscopy
Electron microscopy
Drosophila
Electron Microscopy
Tissue
Transmission electron microscopy
Mitochondria
Organelles
Research Personnel
Centrioles
Kinetochores
Smooth Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Golgi Apparatus
Vacuoles
Cytoskeleton
Microtubules
Gold
Fluorescent Antibody Technique
Microscopes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Immunolabeling of thin sections of Drosophila tissues for transmission electron microscopy. / McDonald, Kent L.; Sharp, David J.; Rickoll, Wayne.

In: Cold Spring Harbor Protocols, Vol. 7, No. 7, 07.2012, p. 838-841.

Research output: Contribution to journalArticle

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