Immunohistochemical study on superoxide dismutases in spinal cords from autopsied patients with amyotrophic lateral sclerosis

Noriyuki Shibata, Kohtaro Asayama, Asao Hirano, Makio Kobayashi

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

A role of mutations in the superoxide dismutase (SOD)-1 gene in the pathogenesis of amyotrophic lateral sclerosis (ALS) has been discussed. To investigate immunohistochemical alterations of SOD in the spinal cord affected with this disease, we examined 3 patients in a SOD1 mutation-associated family with ALS, 20 patients with sporadic ALS and 10 control individuals. Lewy body-like hyaline inclusions (LBHIs) were seen in the anterior horn cells of all the familial patients and 10 of the 20 sporadic patients, while skein-like inclusions (SIs) and Bunina bodies (BBs) were present in the 20 sporadic patients but not in the familial patients. The primary antibodies used for immunostaining were rabbit antisera raised against human SOD1 and SOD2. The anti-SOD1 antibody reacted strongly with all LBHIs of each familial patient and with some LBHIs of each sporadic patient. The cytoplasm of morphologically intact and degenerated spinal cord neurons as well as spheroids seen in the cases examined was only weakly stained by the antibody to SOD1 or not at all. The reactive astrocytes displayed weak to moderate staining for SOD1. The anti-SOD2 antibody strongly immunolabeled the reactive astrocytes and microglia. LBHIs of both familial and sporadic ALS were negatively stained for SOD2. Spinal cord neurons and spheroids of each case exhibited no significant SOD2 immunoreactivity. Neither antibodies reacted with SIs nor BBs. These results indicate that SOD1 may be involved in the formative process of LBHIs especially in familial ALS but not always in that of SIs or BBs, and imply that SOD2 may have no connection with any of these ALS-related abnormal structures.

Original languageEnglish (US)
Pages (from-to)492-498
Number of pages7
JournalDevelopmental Neuroscience
Volume18
Issue number5-6
StatePublished - Nov 1996

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Amyotrophic Lateral Sclerosis
Superoxide Dismutase
Spinal Cord
Lewy Bodies
Hyalin
Astrocytes
Antibodies
Anti-Idiotypic Antibodies
Anterior Horn Cells
Neurons
Mutation
Inclusion Bodies
Microglia
Immune Sera
Cytoplasm
Staining and Labeling
Rabbits
Amyotrophic lateral sclerosis 1
Genes

Keywords

  • Amyotrophic lateral sclerosis
  • Bunina bodies
  • Immunohistochemistry
  • Lewy body-like hyaline inclusions
  • Skein-like inclusions
  • Spinal cord
  • Superoxide dismutase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunohistochemical study on superoxide dismutases in spinal cords from autopsied patients with amyotrophic lateral sclerosis. / Shibata, Noriyuki; Asayama, Kohtaro; Hirano, Asao; Kobayashi, Makio.

In: Developmental Neuroscience, Vol. 18, No. 5-6, 11.1996, p. 492-498.

Research output: Contribution to journalArticle

Shibata, Noriyuki ; Asayama, Kohtaro ; Hirano, Asao ; Kobayashi, Makio. / Immunohistochemical study on superoxide dismutases in spinal cords from autopsied patients with amyotrophic lateral sclerosis. In: Developmental Neuroscience. 1996 ; Vol. 18, No. 5-6. pp. 492-498.
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